宮內缺氧對子代大鼠老年期腦白質的影響.pdf

1、Y￡108778分類號：R74102學校代碼：10392福建醫(yī)科大學碩士學位論文宮內缺氧對子代大鼠老年期腦白質的影響Theinfluenceofintrauterinehypoxiaonthecerebralwhitematteroftheelderlyoffspringofrats接收院(系)：第二臨床醫(yī)學院申請人：王凌星學科專業(yè)：神經(jīng)病學導師：蔡若蔚主任醫(yī)師、副教授呂國榮主任醫(yī)師、教授研究起止日期：2004年8月至2006年11月二

2、零零七年一月TheinfluenceofintrauterinehypoxiaonthecerebralwhitematteroftheelderlyoffspringofratsAbstract[Objects]Tostudythetheinfluenceofintrauterinehypoxia(IH)onthecerebralwhitematteroftheelderlyoffspringofrats[Methods]SDrats

3、intrauterinehypoxiamodelwasestablished，thentheiroffspringwereexposedtohigh—fatdiet(HF)and(or)secondhypoxia(SH)InⅢnunohistochemistrywasusedtodetectedtheexpressionofmyelinbasicprotein(MBP)，neurofilament—HL(NF—HL)，andglialf

4、ibrillaryacidicprotein(GFAP)inthewhitematterbesidethelateralventricleoftheelderlyoffspringMeanwhileMoticpathologicalimageanalysiSsystemwasusedtodeterminethequantityMicrostructuresofthemyelinaxonandmicrovascularintheratbr

5、ainwereobservedbyelectronmicroscopeThree—factortwo—levelanalysisofvarianceWaSappliedtoanalyzetheexperimentaldata[Results]ThemaineffectsofIH，HFandSHallreducedtheexpressionofMBPandNF—H十LandincreasedtheexpressionofGFAPinwhi

6、tematteroftheelderlyoffspringofrats(PO05)InteractionexistednotonlybetweenIHandHF。IHandSH，orHFandSH(PO，05)butalsoamongIH，MFandSH(PO05)Moreover，therewasahighpositirecorrelationbetweentheexpressionofMBPandNF—HLinthewhitemat

7、terbesidethelateralventricle(胙064，PO。01)Moredemyelination，injuredaxon，damagedmicrovasculars，neuronaldegenerationandapoptosiswereobservedbytheelectronmicroscopeinIHgroupthaninthecontrolgroupAndthesepathological1esionsinth