介孔硅分子篩-PVA復(fù)合膜固定化漆酶電極的研究.pdf

1、天津大學(xué)碩士學(xué)位論文介孔硅分子篩PVA復(fù)合膜固定化漆酶電極的研究姓名：周玉梅申請學(xué)位級別：碩士專業(yè)：材料學(xué)指導(dǎo)教師：許鑫華20070601摘要ABSTRACTLaccasebiosensorhascapabilityofdetectingabroadrangeoftoxicsubstratesrapidlyandconvenientlyinwastewaterincludingphenoliccompounds，arylamines，o

2、rganicphosphoniccompounds(pesticide)，dioxins，andSOonSo，itisvaluabletofabricatelaccsemodifiedelectrodewithexcellentpropertiessuchashighsensitivity，lowdetectionlimit，broadlinearrangeandrapidresponsebecauseofitsimportantapp

3、licationinprotectingenvironmentThreekindsofmesoporoussilicasieves，MCM41，MCM41containingM92(denotedhereafterasMgMCM41)andMCM41containingmethyleneBlue(denotedhereafterasMB—MCM41)，weresynthesizedbythemethodsofhydrothermaisy

4、nthesisandwereusedtofabricateexcellentlaccasemodifiedelectrodefirstlywhenpoly(vinylalcoh01)(PVA)wasinpresenceN2adsorption／desorption、SEM、TEMandelectrochemicalmethodswereusedtocharacterizethesemodifiedelectrodesTheresults

5、ofMCM41／PVAimmobilizedlaccasemodifiedelectrode(denotedhereafterasMCM41／PVA／Lac)showedthatoneprotonparticipatedintheelectrontransferprocessonMCM41／PVA／LacmodifiedelectrodesurfaceinpH480PBScontaining005mMcatech01Underoptim

6、izationoftheexperimentalparameters，MCM一41／PVA／Lacexhibitedexcellentresponsepropertiestocatecholandimprovedsensitivity，responserate，linearrange，detectionlimitandSOonMCM一41／PVA／Lachasanattractingapplicationindetectingandin

7、spectingwastewaterTheresultsofMgMCM—41／PVAimmobilizedlaccasemodifiedelectrode(denotedhereafterasMgMCM41／PVA／Lac)showedthatMgppromotedcatalyticoxidationoflaccaseandimprovedelectrontransferbetweenlaccaseandelectrode，moreov

8、er，MgMCM41／PVA／Lacexhibitedbetteraffinitytocatechol，highersensitivity，fasterresponse，broaderlinearrangeandlowerdetectionlimitthanMCM41／PVA／LacMgMCM41providedanovelsupportforiaccasemodifiedelectrodeTheresultsofMBMCM4l／PVA