電滲泵-毫米整體柱電色譜聯(lián)用測定食品防腐劑.pdf

1、中國科學技術大學碩士學位論文電滲泵毫米整體柱電色譜聯(lián)用測定食品防腐劑姓名：高勇申請學位級別：碩士專業(yè)：分析化學指導教師：何友昭20090601ABSTRACTABSTRACTElectrokineticflowanalysis(EFA)systemisbasedonsequentialinjectionanalysis(StA)，multicommutationanalysis，electroosmoticdeliveryandchro

2、matographicseparationTheEFAsystempossessesitsadvantagesofsimplicityconvenience，multifunctionandcontrollabilityThemainaimofthisthesisistoextenditsanalyticalfunctionandapplicationA20mnlidCssilicamonolithiccolumnwasprepared

3、byonesteppolymerizationThepreparationconditionsofthereversedphasesilicamonolithicmicrocolumnfilledwithfinequartzsandanditschromatographicseparationbehaviorwerestudiedInaddition，thepreparedmicrocolumnhasbeenemployedinthes

4、eparationofparabensbyreversedphasechromatographyandelectrochromatography(EC)deliveredbypressure／electroosmosisflowsuccessflly1Silicamonolithicchromatographycolumnsfunctionalizedwithoctylgroupsweresynthesizedbyone—steppol

5、ymerizationfromtetrathoxyailane(TEOS)andnoctyltriethoxsiliane(CsTEOS)hydrolyzedbyacidandpolymerizedunderbaseconditionsTheinfluencefactorsinthesolgelprocedurewereinvestigated，suchasacidicconcentrationandreactiontimeTheopt

6、imalpreparationconditionsarelistedasfollows：05mLTEOS，04mLCsTEOS，06mLethanol，010mL0016mol／LHClweremixedfor1min，hydrolyzedat600Cfor6handcooledtoroomtemperatureN—dodecylamineof25mgwasaddedintothesolgelsolution，andthenthemix

7、edsolutionwasintroducedintoaPTFEmoldandpolymerizedat600Cfor45hThemicromorphologyandelementanalysisresultsofthemonolithiccolumnvalidatedthatthemicrocolumncouldbeemployedinthechromatographicseparationForthepreparationofthe

8、silicamonolithiccolumnmentionedabove，themicrocolumnswerepreparedina20toniidfusedsilicatubefilledwith100120lxmquartzsandtoavoidcolumnshrinkageThreeparabenswereseparatedonbaselineusingthesilicamonolithicmicrocolumnswithamo