垂體發(fā)育相關(guān)基因的篩選和垂體cDNA表達文庫的構(gòu)建.pdf

1、上海第三墾型奎堂墮主嬰窒圭蘭些堡三一————————————————————————————————————一縮寫APBCIPBMPbpcGMPcAMPcDNADEPCDIGDTTEEGFIGFKbpmRNAORFPBSProplSSCMinhrW英文全稱縮略詞對照中文全稱堿性磷酸酶5brono一4chloro—indodylyphosphate5溴4氯一3吲哚酸bonemorhogenticproteinbasepairscyclic

2、guaninemonophosphatecyclicadenosinemonophosphatecomplementDNAdiethylpyroearbonatedigoxinditlliothreitalembryoepidemicgrowthfactorinsulin—likegrowthfactorkilobasepairsmessengerRNAopenreadingframephosphatebufferedsalinepri

3、phetofPit1standardsalinecitrateminutehourweek骨形態(tài)發(fā)生蛋白堿基對環(huán)一磷酸鳥苷環(huán)一磷酸腺苷互補DNA焦碳酸二乙酯地高辛二硫疏糖醇胚胎表皮生長因子胰島素樣生長因子千堿基對信使RNA開放閱讀框架磷酸鹽緩沖液Pit1基因轉(zhuǎn)錄因子檸檬酸緩沖液分小時周圭塑苧三墮型查堂堂主堡窒生望些堡壅SelectionofPituitaryDevelopmentassociatedGenesandConstructio

4、nofThePituitaryeDNALibraryAbstractInordertoexplorethemechanismsofpituitarydevelopmentanditsassociatedgenes，weselected17candidategenesfromthreepituitarycDNAlibrariescontaining8078ESTsand200novelsgenesByusingRNAinsituhybri

5、dization，weobservedtheexpressionofthesegenesinhumanembryo’SpituitaryatdifferentstageanddifferentlocationTheresultshowedthatNKX62，CDX5，TPKA，SKRAB，Wnt一13，XIST，OIFarehighlyexpressedindifferentstagesofthepituitarydevelopment

6、andlocatedwidelyinthedifferentareaofpituitarydevelopmentSOX9，CLlM2，PMX一2，MEIS2，IL一7，CortareweaklyexpressedduringthepituitarydevelopmentNURR一1andPLAGlshowedincreasingexpressionwiththematuringofthepituitarydevelopmentPrhan

7、dHElwerenotexpressedinpituitaryatanystagesandanyareaduringpituitarydevelopmentTostudythemechanismsofthepituitarydevelopmentbyusingmousemodel，wesuccessfullyconstructedtheE165mouseembryopituitaryeDNAlibraryWereportedthe17g

8、enesexpressingduringpituitarydevelopment，amongthemSKRAB、CLIM2、OIF、CDX5、NKX62，PMX一2、TPKAarenovelgenesTheE165mouseembryopituitarycDNAlibraryprovidedausefultoolforstudyingpituitarydevelopmentkeywords：pituitarydevelopment；in