生物粘合與生物礦化啟發(fā)下多酶催化系統(tǒng)的構(gòu)建.pdf

1、天津大學(xué)博士學(xué)位論文生物粘合與生物礦化啟發(fā)下多酶催化系統(tǒng)的構(gòu)建姓名：張蕾申請學(xué)位級別：博士專業(yè)：生物分子工程指導(dǎo)教師：姜忠義201011ABSTRACTInthiswo叱mimickingthemultienzymecoexistenceapproachinmitochondria，o唱anic—ino唱anichybridmicrocapsuleswerepreparedundermildconditio曠／，，temperature

2、，neutralpHandaqueoussolution)throughthesynergybeyweenbioadhesionandbiomineralizationStarchwasconvertedintoisomaItooljgosaccharide(IMOs)byaamylase，pamylase，andglucosidasespatiallyseparatedimmobilizedinbiomimeticmicrocapsu

3、lesThedetailsinthisworkweresummarizedasfolIows：Firstly，thecharacteristicsof0LamyIase，p—amyIaseandglucosidasewereexaminedrespectiVely，andappropriatereactionconditionofmultienzymecatalysiswasexploredConsideringthereactionr

4、ateandenzymestabiIity，themultienzymereactionconditjonwasdete肋jnedasfollows：800KUaamylase，600upamylaseand420KUglucosidasewereaddedintoeve呵kilogramstarch，andthercacitontookplaceinpH60aqueoussolutionat500CUndersuchcondition

5、，theyieldofIMOs疳omStarchwas39％Inspjredbybioadhesionphenomenon，polydop鋤ine(PDA)microcapsuleswerepreparedthrou曲therapidselfpolymerizationofdopamineonthesurfaceofCaC03(PSS)tI鋤pIateundermildconditjonThewallthicknessoftbemicr

6、ocapsuIescouldbetuned盼polymerizationtimeandthedop砌ineconcentrationi11anaqueoussolutionThethreeenzymeswercimmobilizedinthelumen，withinthewallaIldonthe0utsurfaceOfmicrocapsuleundereXtremelymildconditions，respectivelyTheyie

7、IdofIMOsf如mstarchaccomplishedbythemultienzymesyStemwas53％，theoperationstabilit)，ofthemuItienzymesystemismarklyhigherthanthatoffreemultienzymesystemInSpiredbybiomineraljzationandbjoadhesjonphenomenon，anovelapproachofprepa

8、rationo唱anicino瑪anichybridmaterialundermildconditionwaSdesignedThesynthesisofino唱anicoxidewasinducedbybiomacromolecule，followedbydopamineself_polymerizationonthesurfaceofino略anicoxideThoughthismethod，protamine。silica—pol

9、ydopamine(PSi—PDA)microcapsuleswercpreparedforspatiallyseparatedmultienzymecoimmobilizationComparedwithPDAmicrocapsulesandthePSimicrocapsuJes，themechanicalstabilityofPSiPDAmiclocapsuleswassignificantlyimproVedbythesyneI影