夫西地酸發(fā)酵及分離純化工藝的初步研究.pdf

1、上海師范大學(xué)碩士學(xué)位論文夫西地酸發(fā)酵及分離純化工藝的初步研究姓名：鄧昕申請學(xué)位級別：碩士專業(yè)：微生物學(xué)指導(dǎo)教師：陳代杰201203上海師范大學(xué)碩士學(xué)位論文摘要AbstractStudiesonthefermentationandseparationandpurificationofFusidicacidSIPI—A3201isastrain，preservedinourlaboratory，whichmayproducefusidica

2、cidInthispaper，someresearcheswerecarriedoutofwhichincludestrainimprovement，optimizationoftheprocessesoffermentation，separationandpurificationAfterfusidicacidwasdeterminedandverifiedbyHPLCandLCMSinthebrothfromstrainSIPIA3

3、201proservedinLab，naturalselectionandUVmutagenesiswerecarriedoutThehighyieldmutantSIPIA3202，whosefermentationleveloffusidicacidwasuptO180pg／mLTherelationshipoftheproductivityandmorphologybetweenhi曲andlowerstrainswerestud

4、iedTheresultsshowedthattheamountandspeedofsporesfomedduringfermentationwereinverselyproportionaltOthebiosynthesisoffusidicacidFermentationmediumandcultivationconditionwereoptimizedTheproductivityofmutantSIPIA3202underthe

5、optimizedmediumandcultivationconditionincreasedaboutthreetimescomparedtotheparentstrainunderoriginalmediumandconditionFusidicacidWasseparatedandpurifiedfromthebrothaccordingtOthefollowingprocessThepHofbrothwasadjustedtO2

6、3byaddingoxalicacid，andthenthecellswereharvestedbycentrifugationandextractedby95％ethan01TheextractedphaseWasconcentratedandseparatedandpurifiedbymacroporousadsorptionHZ818111efusidicacidwithpurityof90％abovewasobtainedKey