大腸桿菌F5菌毛膠體金免疫層析試紙條的研制及初步應用.pdf

1、Cl韶sifiedIndex:U.D.C:ThesisfortheMasterDegreeinAgricultureDEVELOPMENTANDPRELIM研ARYAPPLICATIONOFTHECOLLOIDALGOLDIMMUNOCHROMATOGRAPHICDETECTINGTECHNOLOGYFORESCHERICHIACOLIFSFIMBRIAECandidate:SuPervisor:AeademieDegreeAPPlie

2、dfor:SPeeiality:DateofOralExsmination:University:YangXudongProf.ShiDongfangMasterofAgriculturePreventiveVeterinaryMedicine五me，2010卜陌曲eastAgricu拓萬alUI如ersity東北農業(yè)大學農學碩士學位論文3.1.1純化后腹水單抗蛋白濃度的測定...............................

3、....……，...............................……233.1.2純化后腹水單抗效價的測定......……，..............................……，...............……，.........……233.1.3純化后腹水單抗純度的檢測.......................................................................

4、..........……233.2兔抗F5血清制備及其抗體lgG的純化....................................……~.....……，………243.2.1FS蛋白的誘導表達及其純化..........……，...............................................……，.........……243.2.2純化后兔抗FS血清抗體蛋白的測定..................

5、.................................................……243.2.3純化后兔抗FS菌毛血清抗體效價的測定...........................................................……243.2.4純化后血清多抗純度的檢測.…，.…，..…，..…，..…，............……、…‘二、...........................

6、……243.3幾種常用方法對FS菌毛的檢測..................................................................................……253.3.1間接夾心ELIsA檢測.........................................……253.3.2平板凝集檢測.........................……，.…，..........

7、....................，……263.3.3PCR檢測......................……，...................................................................................……263.4膠體金質量的評價..............................................................

8、.............……273.5免疫膠體金探針的制備...........................................一283.5.1膠體金吸附抗體蛋白最佳pH值的確定..............................................................……283.5.2抗體結合膠體金最佳蛋白濃度的確定.‘二，...……、.........................

9、........……，.........……283.5.3免疫膠體金探針的檢測.............................……293.6免疫層析反應條件的優(yōu)化................................，……303.6.1試紙條組裝材料的選擇....................................……303.6.2緩沖溶液的優(yōu)化..............................

10、......................................................................……303.7膠體金免疫層析試紙條的組裝....................................................................................……313.7.1組裝后試紙條的結果判定說明........................

11、....................................................……313.7.2試紙條性能的測定二，.............................................................................................……323.7.3試紙條的初步應用.……、.‘二、、、.............................

12、.....................................................……344討論......................................................................................................................................……354.1影響膠體金檢測試紙條制備的幾個關鍵技術

13、環(huán)節(jié)....................................................……354.1.1膠體金溶液的選擇...............................................................................................……354.1.2金標單抗的制備.................……，..................

14、...........................................................……354.1.3試紙條各組分和材料的選擇優(yōu)化........................................................……，.........……364.2檢鋇l樣品的選擇.......................................................

15、....................................................……375結論.........................................................................................……，......................................……38致謝.........................

16、.................................................................................................................……39參考文獻...................................................................……，二、.....……，.、，................