蛋白質(zhì)折疊，結(jié)構(gòu)拆解和結(jié)合部位多樣性的研究.pdf

1、F794780中圖分類碼：Q61，Q5182專業(yè)分類碼：081704學(xué)科分類代碼：1807130天津理工大學(xué)碩士學(xué)位論文蛋白質(zhì)折疊，結(jié)構(gòu)拆解和結(jié)合部位多樣性的研究(申請碩士學(xué)位)學(xué)科專業(yè)：應(yīng)用化學(xué)研究方向：生物信息學(xué)研究生：田晶指導(dǎo)教師：黃積濤教授二零零五年一月Studiesofproteinfoldingmechanism，structuralsplittingandbindingsitediversityAbstractTherea

2、rethreeinformationsoarcesofproteinscienceinbioinformatics：sequence，structureandfunction111eproblemofproteinfoldingbelongstotherelationshipbetweenproteinsequenceanditsstructure；andtheproblemofrelationshipbetweenproteinstr

3、uctureanditsbiologicalfunctionaleoneofthelargestchallengesinmolecularbiologyStructuraldeterminantsofproteinfoldingmechanismwerestudiedWeassumedthatthereisarepulsivepotentialbetweenaminoacidresiduepairsinproteinstructureW

4、henproteinfolds，theirconformationcollapsesAresidueliesclosetoanotherresidueandSOwouldcausesterichindrancetolutherfoldSothefoldingratedecreasedquicklywitllanincreasingmagnitudeoftherepulsivebarrierTwodifferentmathematical

5、formulaswcfeusedtodescribethepotentialfunetionOneisanequationbased011Lennard／onesformulawhichthepotentialiSinverselyproportionaltesiduedistancetothe65thpower(E=1，礦3、。TheotheriSsimilartoMorseformulaandthepotentialdecrease

6、dexponentiallywithincreaseinthedistance忸=e156)【d)66twostateproteinsmultistateproteinsandshortpeptidewereanalyzedstatistically11他resultshowsastrongcorrelationbetweenthepotentialsandfoldingrates，whichcorrelationeoefficiema

7、rcO80and081，t刪ve1yContactresidues趣ethedeteminantsofprotein—foldingkineticsProtein嗣nKtl∞∞，especiallytertiarystructures，arecloselyrelatedtotheirbiologicalrolesHoweveritisdifficulttodistinguishbetweensecondarystructureandte

8、rtiaryoneHere，thebackbonestructureofaproteinWassuppressedthenoiseofhi【ghfrequencyandfoldingpatternoflowfrequencyWasreserved11Iisimpliesthatsecondarystnlctureisremovedfromproteinstructureand“pure’tertiaryslructureisobtain

9、edThjsapproachhighii曲tsthelongrangestructureofproteinandCanprovideadatasourceforpredictionofproteinstructttreItisverydifficulttosuperposethespatialstructuresofproteinsinstudyoftherelationshipbetweenproteinstructuresandth

10、eirfunctionsWehereinselectedporphyrinbindingmyoglobinproteinswhicharedifferentinsequenceandstructure‰msuperpositionofglobalstructuresofproteinsWasperformedusingligandsupcrpositionStructuraldiversityofaseriesofproteinsiss