哺乳動物病毒型TLRs基因的適應(yīng)性進化及鯨類TLR8的功能驗證研究.pdf

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2、紙質(zhì)文檔，可以借閱或上網(wǎng)公布本學位論文的部分或全部內(nèi)容，可以采用影印、復(fù)印等手段保存、匯編本學位論文。學校可以向國家有關(guān)機關(guān)或機構(gòu)送交論文的電子和紙質(zhì)文檔，允許論文被查閱和借閱。(保密論文在解密后遵守此規(guī)定)保密論文注釋：本學位論文屬于保密論文，密級：——保密期限為——年。學位論文作者簽名：萄準鬲指導(dǎo)教師簽名：么名徨淬Et期：00“!2中Et期：oo形‘之_AbstractMammalsarethehighestformofverteb

3、ratesandholdadominantpositionontheearthMostofthemarewithhaitconstanttemperatureandavarietyoffeedinghabitsThelivingenvironmentsofmammalsarevariousincludingterrene，ocean，skyandcaveDuetothevastanddiversityofmammals，somespec

4、ialgroupsofthemareboundtohappensignificantlydifferentadaptivechangeInnateimmunity，asasystemthatmostanimalsuseagainstpathogenicmicroorganisminvasion，isrelativelyoldandthebody’Sfirstlineofdefenseagainstpathogenicmicroorgan

5、ismsTolllikereceptors(TL黜)belongtoanoldfamilywhichmediatesinnateimmunity，dividedintoviralTLRsandnonviralTLRsAmongthem，TLR3，TLR7，TLR8andTLR9belongtoviralTLRsTheyarelocatedonthemembranesofendosomes，andidentifytheviralnucle

6、icacidsBecauseofthegreatdifferencesinlivingenvironmentsandfeedinghabitsofmammals，theviruses’typesarealsodifferentHowevertheresearchontheevolutionmechanismofviralTLRsiSgenerallyconsideredtobeconservativeToexplorethepossib

7、lemolecularmechanismoftheevolutionofmammals’viralTLRs，TLR3，TLR7TLR8andTLR9werechosenforevolutionaryanalysisill27representativemammalsTheselectivetestimplementedwithSiteModel，BranchsiteModelinPAMLandSLAC，F(xiàn)EL，RELillDatamon

8、keydiscoversagreatnumberofpositiveselectedsitesinthefourviralTLRsofmammalsandthemostinTLR9MostoftheSepositiveselectedsitesarelocatedintheLRRdomainintheextracellulardomainLRRdomainistheregionwhichdirectcombinewithPAMPs，an

9、dhasbeenunderstrongpositiveselectionThroughtheanalysisoftheBranchsiteModelandfleeratiomodel，itisshowedthatviralTLRshasbeenunderstrongpositiveselectedpressuresiIlcetaceansandChiropteraThisindicatesthatthetypesandnumberofv

10、irusesofcetaceansandChiropterahavechangedduetothemarineenvironmentandtheskycavesenvironmentiIlthelongprocessofevolutionandtheselectedpressureoftheirviralTLRshaschangedtooWealsofindpositiveselectedsignalsintheotherbranche

11、ssuchasCetartiodactylaandPrimatesItisshowedthemammalianviralTLRshavebeenunderuniversalpositiveselectionWedetectedthemostcommonpositiveselectedsignalsinTLR9，suggestingthatitmaybetheonlyTLRtoidentifytheviralDNAThismayberes