生物微膠囊固定化溶栓酶產(chǎn)生菌及釀酒酵母微囊化培養(yǎng)模型化的初步研究.pdf

1、浙江大學(xué)碩士學(xué)位論文生物微膠囊固定化溶栓酶產(chǎn)生菌及釀酒酵母微囊化培養(yǎng)模型化的初步研究姓名：姚勁挺申請學(xué)位級別：碩士專業(yè)：生物化學(xué)工程指導(dǎo)教師：梅樂和2001.1.1浙江大學(xué)碩士學(xué)位論文ABSTRACTABSTRACTFreeandimmobilizedcultivationofBacillussubtilisgenu(ZJUBETI)whichcanproduceantithrombusenzyme(ATE)wasstudiedAfun

2、damentalmathematicmodelaboutproductionofethaneIbySaccharomyccseerevisiaeimmobilizedinNaCSPDMDAACmicrocapsuleswasestablishedTheinfluenceoffreecultureonproductionofATEandgrowthofbacteriawereinvestigatedWhenglucose(05％)Wasa

3、ddedintoLBculturemedium，thepropagationofbacteriaWasprohibitedbecausepHdecreasedsignificantlyTheoptimumconcentrationofphosphateWasO30％(NaI12P042H20)and144％(Na2HP0412H20)Basedontheconcen仃afionofbufferahead，theoptimumconcen

4、trationofglucoseWas05％ThecharacterofgrowthofthegenuWasstudiedwhenammoniBillsulfateorureawasusedasnitrogensoRreeWhenhydrolyzedtryptoneservedasni仃ogensource，也eenzymeactivityreached757UK枷ThecharacterofproductionofATEbythege

5、nuencapsulatedinNaCSPDMDAACmicrocapsuleswasstudiedTherelationshipbetweenairvolumnvelocityandmeandiameterofmicrocapsulesintheprocessofproducingmicrocapsulesWasstudiedfirstiyThentheinfluenceofmediumdiameterofmicrocapsulesc

6、oncentrationofPDⅣ【DAACandthecomponentsofculturemediumonproductionofA：rEintheconditionofencapsulationcultureWasstudiedInshakingflasks，themaximumenzymeactivityinmicmcapsulesWas1850UK／111l(urokinase卸油i鉀uniteachmilliliterbro

7、thinmicrocapsules)whentheairvohmmvelocityWas03m3／h(themeandiameterofmicrocapsulesWas160ram)theconcentrationofPDMDAACwas20％andtheculturemeAiumwasLBculturemedium(tryptone)glucose(o。5％)phosphatebuffer(100m1)Abubblingculture

8、systemWasdesignedInthissystem，themaximumenzymeactivityinmicrocapsulesreached2050lJ剮m1whichWas36timesthatinsuspensionfermentation；themaximumeelldensityinmicrocapsuleswas489109cell／m1whichWas27timesthatinsuspensionfermenta

9、tionAsimplemathematicmodeldescribingproductionofethanolbySaccharomycescerevisiaeimmobilizedinNaCSPDMDAACmicrocapsulesWasestablishedn地mainreasonsthatcauseddeviationbetweenexperimentalvalueandcalculatedValliewerediscusseda