蘇云金芽胞桿菌菌株CT-43產(chǎn)蘇云金素及其缺失突變株的差異蛋白.pdf

1、華中農(nóng)業(yè)大學(xué)碩士學(xué)位論文蘇云金芽胞桿菌菌株CT43產(chǎn)蘇云金素及其缺失突變株的差異蛋白姓名：郭承亮申請學(xué)位級別：碩士專業(yè)：微生物學(xué)指導(dǎo)教師：喻子牛孫明20080601華中農(nóng)業(yè)人學(xué)顧l：學(xué)位論義2008AbstractThuringiensinisanonspecificheatstableinsecticidaltoxinproducedbysomeBacillusthuringiensisstrainsThisexotoxinishea

2、tstableanditschemicalstructureissimilartothatofnucleotides，itsmelecularweightis701ThemechanismofinsecticidalactionistoinhibittheproductionofDNAdependentRNApolymerasebycompetitionwithATPSOithasabroad—spectrumofeffectivity

3、againstmanypestsThetoxicityofthuringiensinismuchlessthanthatofmostchemicalinsecticides；thereforeitshowsgreatpotentialtobecomeaveryusefulinsecticideforawiderangeofpestsInordertoclarifytheinfluentialrolefactorofbiosynthesi

4、sandmetabolicofthuringiensinproductiondifferentialexpressionanalysisbetweenthehi曲thuringiensinyieldBacillusthuringiensisstrainCT43anditsmutants，highproductionstrainCT431CandnonproductionstrainF15isconductedbasedonthe2一Dg

5、elThenthroughmassspectrum(MS)identificationandbio—informaticstoanalyzethedetectedproteinsThirteenspotswereselectedtobedetectedbythemassspectrum，andnineofthemweresuccessfullyidentifiedfinallyAmongw11iehsixproteinsincludin

6、ginthebasalmetabolismpathwayarepossiblydeducedthatassociate、析mthesynthesisofthuringiensinproductionThedeletionofdihydroxyacetonekinaseandNacetylglucosamine6phosphatedeacetylase，ortheimprovementofAconitatehydratase1mayaff

7、ectthemetabolismpathwayofThuringiensinAndonenecessaryproteinUvrB／UvrCbelongingtothesecretionpathwaywasspeculatedtoberelatedtothethuringiensinproductionandsecretionwayandoneproteinnamedphoHproteinWasdeletedinthemutantstra

8、in，whichwasdeducedtoberelatedtothethuringiensinproductionsynthesis’Smetabolismsubstrateaswellastheenergymetabolism；leucyltRNAsynthetasereduced，whichprobablycausedthatBacillusthuringiensisstrainF15didnotproducethuringiens

9、inInthisresearch，TherearesixproteinfoundtobeconnectedwiththeinfluentialroleproteinofbiosynthesisandmetabolicofthuringiensinproductionbycomparativeproteomicsAndthisresearchprovidesasupportingevidenceofthuringiensisgeneclu