擬南芥核糖蛋白基因RPL36B在外源體系——胡蘿卜及酵母中的表達(dá).pdf

1、分類號(hào)UDC密級(jí)學(xué)位論文型直薟茲蕉蛋白基國恥豎鯉查生I漚簽囂ExpressionofArabidopsisRibosoma—lProteinGeneRP玢6BinCarrotandYeast勉指導(dǎo)教師姓名董國俊副熬援遺昌去堂壘金登堂堂筮申請(qǐng)學(xué)位級(jí)別理堂遁專業(yè)名稱壘塑些堂：皇盆王生趁堂論文提交匿麓2魚Q魚堡三旦論文答辯日期2立立量璽壘旦基學(xué)位授予單位和日期菹基盍鱟2Q璺晝生Z題旦答辯委員會(huì)主寒評(píng)閱人2006年5月日AbstractRegu

2、lationofgeneexpressioncanoccuratdifferentlevels，forinstance，initiationoftranscription，splicing，stablityofRNA，translationandmodificationofproteinandsoonEverylevelisveryimportanttothenormalgrowthofcell5’TOP(terminaloligopy

3、rimidine)mRNAspeciescontainastretchofoligopyrimidinesatitsvery5’end；predominantlyencodefortranslationfactorsandribosomalproteinsubunitsTherehasbeenextensivestudyintheregulationof5’TOPmRNAinvertebrate，buttherehasn’tbeenan

4、yreportaboutsimilartranslationalcontrolonplantribosomalproteinmRNAsInthecarrotgenome，therearethreedifferentcopiesofRPL36genesencodinganidenticalpolypeptideItwillbedifficulttodistinguishthemTherefore，wechooseArabidopsisri

5、bosomalproteingeneRPL36BtobeexpressedincarrotasanexampletoinvestigatethephenomenonandmechanismoftranslationalcontrolonplantribosomalproteingenesByintroducingArabidopsisribosomalproteingeneRPL36Bintocarrottogeneratetransg

6、eniccelllines，itispossibletostudythemechanismsoftranslationalregulationoftransgenicArabidopsisribosomalproteingeneRPL36BincomparisonwiththeendogenouscarrotRPL36mRNAsindevelopingcarrotembryosThisthesisfocusesontheprocedur

7、esoftransformationofArabidopsisRPL36Bgeneintocarrotandyeast，detectionofthetransformedgenesinthehostceils，andexaminationoftheexpressionofthetransfectedgenesindifferentsystemsFromthisworkastandardprotocolofmakingtransgenic

8、plantanddetectionoftheexistenceandexpressionofthetransgenewereestablishedTransformationofcarrotwasmediatedbyAgrobacteriumtumefaciensGV3101andtransformationofyeastwasachievedbychemicalmethodByusingX—Glucstaining，PCR，andRT

9、PCR，wewereabletoconfirmexpressionoftheArabidopsisRPL36BgeneintransgeniccarrotandinyeastInterestinglytheresultsindicatethatArabidopsisRPL36BgeneisdifferentiallyexpressedincarrotandinyeastBesides，weperformed5’RACEcombinedw