抑制衰老的嵌合基因在百脈根及野牛草中的轉化研究.pdf

1、弋蛾寰f文等‘博士學位論文Y940057抑制衰老的嵌合基因在百脈根及野牛草中的轉化研究StudiesonTransformationofaSenescenceinhibitionChimericGeneintheLotusandBuffalograss研究生：王瞳苤指導教師：旦丞熬援合作指導教師：申請學位門類級別：壅堂譴研究方向：筮塋疊王!筮堇直弛厘生物掛苤所在學院：動物型堂撞盔堂瞳本論文資助項目：國家轉基因植物研究與產(chǎn)業(yè)化專項“優(yōu)質、

2、抗逆草坪草新品種培育”(JY03B。3102)2006年6月AbstractEffectoflotusexplantssourcesoncallusinductionfrequencyandregenerationrateandtherestrainingsenescencegene，PSG529transformationonbuffalograssandlotusrespectivelywithparticlegunandCO—cu

3、ltivationwithAgrobacteriumtumefacienswerestudiedinthisarticleTheresultsshowedthat：1Theefficientregenerationtransformationsystemoflotnshadbeenestablished：Thepreferablyhighcallusinductionfrequencyandgoodqualitycalluswereac

4、quiredfromthestemfragmentsoflotus“LiAo”ontheMS01m班6BAsubculturedevery1822days2ThelotusplantstransformedPSG529wereobtainedbymeansofAgrobacteriummediatedIransformation3ThedetectionresultsfromPeRanalysisofNPT11geneshowedtha

5、tthroughseveralturnsofantibioticsfiltrationthemosttransformedplantscontainedobjectivegeneThisindicatedfiltrationsystemintheexperimentscouldbasicallypreventfmasculineplantsandembedbody4TheresultsofdotblotsandSouthernblots

6、forobjectivegenedemonstratedthattheIPTgenewithspecialpromoterhadbeentransformedintolotusThetransformationratewas58％5TheCTKandchlorophyllcontentsoftransgeniclotusplantsshowedthatthetransformedPSG529genehadworkedonleafsene

7、scenceintransgeniclotusplants6PSG529withspecialpromoterwastransformedintothebuffalograssbygenegun，andwereconfirmedbyPeRdetectingtheNPTIIgeneKeyWords：lotus；buffalograss；senescence。inhibition，gene’guntransformation，agrobac