微流控芯片與激光拉曼光鑷系統(tǒng)聯(lián)用技術(shù)的研究.pdf

1、廣西師范大學(xué)碩士學(xué)位論文微流控芯片與激光拉曼光鑷系統(tǒng)聯(lián)用技術(shù)的研究姓名：艾敏申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：理論物理指導(dǎo)教師：劉軍賢;姚輝璐20090401ii Study of the Combination of Microfluidic Chip and Raman Tweezers Student: Ai Min, Supervisors: Liu Jun- xian, Yao Hui- lu Major: Theory

2、 Physics, Study field: Biologic Physics, Entrance Year: 2006 Abstract Raman tweezers is an optical technology coupled optical tweezers and Raman spectroscopy, also called the Raman spectroscopy co

3、mbined with optical trap (RSOT) technique or the laser tweezers Raman spectroscopy (LTRS) system. A laser beam is introduced into an inverted microscope through a high numerical aperture objecti

4、ve to form an optical trap termed optical tweezers. The optical tweezers can trap a single particle in three dimensions nearly in the balance of the focus of laser beam. The same laser beam

5、 is used to excite Raman scattering of the trapped particle. The scattering light from the particle is collected by the objective and coupled into a spectrograph. With the analysis of the Ra

6、man spectra data, different particles or cells can been recognized and distinguished. It is one of the hot tools on single cell-molecular level in biology field. Microfluidic chips are molecul

7、ar analytical tools for solving the complex analytical challenges associated with the field of biology. Last decade, microfluidic chips have been advanced sharply, which can be named micro- total analysis

8、systems (µ- TAS), lab- on- a- chip or integrated chemistry lab and so forth. Under ideal condition, µ- TAS can intergrate very course of conventional chemical experiments in the laboratory

9、 in the same microdevice, which also is a recent technology in current science study. Microfluidic Raman tweezers combined these two techniques offers a new tool for manipulation and identification of singl

10、e biological cells and microscopic particles automatically. This thesis includes six chapters. Chapter 1 is an introduction, including the history, theory, application and current study of Raman tweeze

11、rs. The main contain is the theories of Raman spectroscopy and optical tweezers, and the application of their combination in biology. The second chapter is the study base of microfluidic chips

12、, mainly containing basic character of microsize, the material and facture of microfluidic chips, and the facture, placement and cleanout of the chip of our laboratory. The third chapter is th

13、e device of Raman tweezes, including laser source, detector, coupled optical path, and so on. The fourth chapter is the capability test of Raman tweezers, including the experiments of rat single cells

14、and polystyrene beads. The fifth chapter is the advance and integration of Raman tweezers, mainly introducing the integration and application of microfluidic Raman tweezers. The sixth chapter is