鹽脅迫和堿脅迫對星星草體內(nèi)檸檬酸代謝相關(guān)酶活性的影響.pdf

1、學撩代碼：!Q!她分樊號：鰓4◎88919《研究生學號：l罌』2QQQ密綴：萋東壯嶧葒失孽碩士學位論文鹽脅迫和堿脅追對星星草體內(nèi)檸檬酸代謝穰關(guān)酶活性妻影響EffectsofNaClandNa2C03stressesonEn料mesAc姍itiesRelatedtoCitrateMetaboIigmi矗A∞曲z蝴渤姍‘‘口勛瑚作者：楊亞軍指導教師：石撼成教授學科專妊：植嘞學研究方向：植物生理學位類趔：學歷碩士東j￡師范大學學位評定委員會2

2、006年5月Abstract尸“cci矸ef矗Ⅱfen“妒ordisatopicalalkalitoleraInplamgrowinginsongnenPltdnlocatedintheNonh—eastareaofChillawheremeso訂isexcessivealkalizationSomeresearchersfoundJp“ccmPf，抽，P玎“f肋，盯speciallyaccunlulatedci仃ateintheSho

3、ot眥deralkaline(Na2C03)仃eatlllemafterseveralhHcncemealkalit01erantplantP“ccf門口盯蛔胞““驢％Mwasselectedasmaterialandci們cacidaccumulationaIldmetabolismunderalkalistressinitwereinvest譫atedW色analvzedfourer因rmesactivi_七iesassociate

4、d謝thcitratemetabolismofP把n“f加rⅡshootandrootu11dersalt(NaCl：0500mM／LN礦)a11dalkaline(Na2C03：O一500mM／LNa十)respectively：citratesynthase(cS，EC41t37)，NADPisocitratedehy出ogenase(NADP—IcDH，ECl1142)，phosphoenolpymvatecarboxylase口

5、EPCase，Ec41131)a11dmalatedehydrogenase(MDH，EC137)Resultsindicatedsalta＆ctedo蟛捌cacjdmetabolismnegligibl弘eitherintheshootormotof尸耙玎“咖r盯(hi曲s訂essimensi夠inducedICDHa11dPEPCaseinrootto10seactivitieslA1kalineiIlducedCSandPEPCa

6、seactivitiesintheshootof戶陀船甜咖Mimproved，especiallycitratesyInhaseactivityenllallceddistinctly；inoppositeNADPICDHactivitydeclinedAstoMDH，itwereuIla任bctcdoralterednegligibl弘UnderlowalkalistressintensitiesCSa11dPEPCaseactivi

7、tiesintherootwereast11esallleasintheshoot，whereasWhenitreachedsonleextent，stressinducedtheroott0d鋤agetolowacitivityasstressiments時wemon，acitivityofIcDHinmerootdrasticlydeclinedfollawingsomeup—gmdeatt|lebeginning，MDHwasno

8、taf免ctedResultsdemons廿atedalkalineimpmvedCSandPEPCaseactivitiesandsuppressedIcDHactivityinPf已胛“棚D陽shootcoincided、]lrithci廿atecomentrosein戶f8刪擴如，dshoot，indicatingalkalineinducedci們teaccmuIadoninttleshootisrelevmltwiⅡlCS，P

9、EPCaseactivitiesincreasea11dICDHactivitydecreaseintlleshootAdjustmemstoCS，ICDHandPEPCaseac“vitiesarebiochemicalbasisofsDeciallvcitratcaccumulationmtheshootofPfP玎”批，ⅢuIlderalkaline(Na2C03)treatmentKeywords：P招”“∥70r口；alkal