產甲烷細菌的分離及其生理學特性的初步研究.pdf

1、貴州大學碩士學位論文產甲烷細菌的分離及其生理學特性的初步研究姓名：張萬芹申請學位級別：碩士專業(yè)：微生物學指導教師：康冀川20080501★貴州大學2008屆碩士學位論文★AbstractMethanogensisaspecialprokaryoticorganismwhichproducelargequantitiesofbiogasastheendmumberthatexistinwLriousextremistanaerobicen

2、vironmentstoproduceenergyAsthefinalmemberofanaerobicbiologicaltreatmentinnatllralc翹Jooncycle，itwansformlargenumberoorganicmatterintenewableenergywithotherflorabacteriassynergyItplayimportantroleinpromotingthecycleofthena

3、ttl托materialandsolutionfortheenergycrisisMethanogensistheCOreofmethanefermentationdevelopmentthebiogaswhichisthekeytosolutiontheruralenergy，Sothecontentsofmenthanogensinthefiredamppoolandthegrowthdirectlyimpactonthequali

4、tyofgasThisstudysimplysummariedthebiomassandbiogasfermentationwhichcloselyrelatedwithmethanogens，andthemethanogens’roleinmethanefermentation(anaerobicdigestion)、probablysummariedit’Sphysiologicalcharacteristicsandecologi

5、calcharacteristics、it’sresearchprogressandresearchsignificanceAccordingtothemethanogens’Smetabolismandthegrowthcharacteristicsintheenvironmentthestudyoptimizethemediumandthespecificseparationmethods，obtainedthemediumandt

6、heseparationmcthodswhicharesuitableformethanogens’SgrowthTheculturemediumwithamixtureofchemicalsWaStestedfortheisolationofmethanogenicbacteriaAboutthemethodsThefirsttimetousethesmallinvertedglasstocollecttllegaswhichispr

7、ocessedbymethanogens，andasthebasistodeterminewhetherthereisanymethanogengrowth，thefirsttimetoanalysethemembolitesiag麒licatsThisstudyobtainedfivepuremethanogenicbacteriasbyusedtheoptimalmediumandseparationmethodaccordingt

8、otheirmorphologicalandbiochemicalcharacteristics，Initiallyidentifiedastheacidm礎anebacillispAndfirsttoanalysethecompositionofthemetabolites，obtainedmethanogenicbacteria’scontentofthemethanewhichismorethan98％Accordingtothe

9、growthofit’sfean鵬，thisstudyreportedthemethanogenicbacteria’SpreservationmethodsInthisstudyfivemethanogens’growthmatrix、PHvalue，temperature，tolerancethesaltconcentrationandpenicillinconcentrationwarestudiedfivestringmetha

10、noheusarcusedonlyacidsodiumforgrouth，theyoptimumgrowthrangeofPHvalueare60t080，Theoptimumgrowthtemperatureis37℃thetolerancetothelargestsaltconcentrationof2％t04％，Andthemostresistanttopenicillinconcentrationis128t0256millio