介體二點(diǎn)葉蟬與玉米鼠耳病的關(guān)系研究.pdf

1、西南農(nóng)業(yè)大學(xué)博士后學(xué)位論文介體二點(diǎn)葉蟬與玉米鼠耳病的關(guān)系研究姓名：劉映紅申請學(xué)位級別：博士后專業(yè)：農(nóng)業(yè)昆蟲與害蟲防治指導(dǎo)教師：@20040601SummaryAdoptingcomprehensivelytheknowledgeandtheoryofpopulationecologyandphytopath高logybythefieldinvestigationsandindoortrials，thebio—ecologicalchar

2、acteristicsandthepathogenicityofCicadulinabipunctella(Mats)，thephytopathogicdetectionandfielddynamicofmaizewallabyearvirus(MWEV)werestudiedsystematicallyTheresultsaresummarizedbelowCbipunctellaisamajorvectorinsecttransmi

3、ttingmaizewallabyearvirus(MWEV)，thedevelopment，survivorship，madfecunditywereevaluatedatdifferenttemperatureandhosts，andthetemporalandspatialdynamicswerestudiedsystematicallyTheresultsindicatedthateggsofCbipunctellafailed

4、todevelopat15℃3rdnymphfailedtosurvivalat35。CandtheoptimaltemperaturerangeforCbipunctellapopulationgrowthwas2528。C’FhemostsuitablehostplantsforthedevelopmentandreproductionofCbipunctellaweremilletandcornamongcorn，wheat，so

5、rghum，rice，millet，andbarleyThedensityofthenaturalpopulationhadthemaximvalueatMay，thespatialdynamicsindicateduniformity(KO)inMaythemdispersal(K0)againfromthelasttendayinJunetothefirstten—dayinJulyThecharacteristicsofebipu

6、nctellacausingMWEVwereconductedwithbioassaymethodTheresultsshowedthat，thevirusofMWEVCanbetransmittedeffectivelytothedescendantsthrougheggs，thetransmissionrateoffemalesarehigherthanmales，thetransmissioncapacityofadultsdec

7、linedafter1012generations，the4thinstarsnymphhasthehighestabilityofcausingdisease，themtheadultandthe5stinstarsnymph，10minaretheminimuminoculation—accessperiodThevirusparticlessimilar、ⅣitIlMWEVwerefoundedinthealvineofCbipu

8、ncteltawhileitCan’tbefoundinothertissueundertheelectronmicroscopyTheabsorbedvaluesofUVspectrophotometers(A260／A280)showed21forthepurifiedvirus，theconcentrationofthevirusis16mg／ml，andtheproductionofroughmaterialisO05ug／gT

9、heDAS—ELISAmethodforMWEVdetectionwasestablishedusingAntiserumTheantiserumandtheantibodywereusedwithadilutionof1：1000and1：5000sequentiallyinthemethodTheDAS—ELISAmethodcoulddetectMWEVinChenopodiumquinoextractatmaximdilutio