線粒體鈣單向轉運體對線粒體通透性轉換孔的調(diào)控作用.pdf

1、青島大學碩士學位論文線粒體鈣單向轉運體對線粒體通透性轉換孔的調(diào)控作用姓名：吳秀云申請學位級別：碩士專業(yè)：麻醉學指導教師：王士雷20120602AbstractobjectiveToinvestigatewhethermitochondrialcalciumuniporter(MCU)cailregulatethemitochondrialpermeabilitytransitionpore(mPTP)inprimaryhippocamp

2、alneuronSMethodsThehippocampalneuronswererandomlydividedintosevengroupsaccordingtotheRandomnumbertable：①controlgroup(n=6)：withoutanyprioradministration；②Sperminegroup(n=6)：gave30panol／LSperminebeforethetest；③Ru360group(n

3、=6)：gave10Imaol／LRu36030minbeforethetest；④CiclosporinAgroup(n=@gave02ttrnol／LCiclosporinA30minbeforethetest；⑤Atractylosidegroup(n=6)：gave400I上mol／LAtractylosidebeforethetest；⑥Ru360Atraetylosidegroup(n=6)：gave10ttmol／LRu3

4、6030minbeforethetestandthengave400panol／LAtractylosidebeforethetest；⑦CielosporinASperminegroup(n=6)：gave02prnol／LCiclosporinA30minbeforethetestandthengave30lamol／LSperminebeforethetest200pmol／LCaCl2wereaddedtoeachgroupat

5、thetimeofthetestbegantoestablishedthemodelofcalciumOVerloadThenmonitoredthemitochondrialcalceinAMfluorescenceintensitycontinuouslyovertimefor6minutesunderthelaerconfocal●‘microscopeResultsThemitochondrialcalceinAMfluores

6、cenceintensityofthecontrolgroupreducedrapidly(00730011)；comparedwiththecontrolgroup，themitochondrialcalceinAMfluorescenceintensityoftheRu360group(O779士0015)andCiclosporinAgroup(O923士0011)reducedslowly滬O05)Compared麗mtheSp

7、erminegroup(0023士0006)，themitochondrialcalceinAMfluorescenceintensityof位Ciclosporin’ASperminegroup(0713士0032)reducedslowly儼O05)；themitochondrialcalceinAMfluorescenceintensityoftheRu360Atractylosidegroup(O643士0087)wasslow

8、erthantheAtractylosidegroup(O047士0015)伊O05)；themitochondrialcalceinAMfluorescenceintensityoftheRu360group(0779士0015)WasslowerthantheSperminegroup(PO05)ConclusionsMitochondrialcalciumuniportercouldregulatethemitochondrial