甾體皂甙的糖苷化反應(yīng)研究.pdf

1、華東師范大學(xué)碩士學(xué)位論文甾體皂甙的糖苷化反應(yīng)研究姓名：周永達(dá)申請學(xué)位級別：碩士專業(yè)：有機化學(xué)指導(dǎo)教師：張劍波20070501ABSTRACTStcroidalsaponinsarecomplexglycosidesofsteroidsand、耐delydistributedinnature，especiallyintheplantkingdomTheyaretheactiveingredientsinmanymedicinalplant

2、sHowevertheamountofsteroidalsaponinsinplantisratherlimited，hinderingitslargescaleofindustrialproductionOntheotherhandthecurrentdocumentedproductionofthesyntheticsteroidalsaponinshasemployedexpensivereagentsandmultipleste

3、pprocesses，whichisunfavorableintermsofgreenindustrialproductionTherefore，enzymaticsynthesisofsteroidalsaponinsshouldbeawayoutTofacethelagofadequatemsearchinthisareathemaintaskofthisthesisistoexplorethesynthesisofsteroida

4、lsaponinsintwosections：1)ChemicalsynthesisofSteroidalsapogenins；2)Explorationofetmynla：ticsynthesisofsteroidalsapogenins1ChemicalsynthesisofsteroidalsapogeRius：Sixcommonglycosyldonorsandthreeaglyconereceptorshavebeenchos

5、enandaseriesofmonosaccharidessteroidalsapogeninshavebeensynthesizedbasedonclassicalKoenigs—KnorrglycosylationalmethodsAllsynthesizedsteroidalsaponinshavebeencharacteafzed，includingtkreecompoundsnot印pearadinthepreviouslit

6、erature2Explorationofenzymaticsynthesisofsteroidalsapogenins：Inthispaper，ourmainconcemistoexplorerhatrmosidaseinthesynthesisofsteroidalsaponins，aftermeticulousexperimentexplorationwefoundrhanmosylCannotbetransferredtotri

7、llinby—rhanmosidasethatfromplantsormicroorganismsInenzymaticsynthesisprocess，wequantitativecharacterizetheactivityoftheplantrhanmosidaseundertheorganicsolventconditionsforthefirsttime，meanwhile，wealsoconfirmedbyexperimen

8、tstheplantrhanmosidasecannottransferglycosyltomacromoleculessuchthillinbutCanshifttosmallmoleculealcohol，andindirectlyconfirmedthespecificoftheplantrhamnosidaseOfcourseourworkabouttheenzymaticsynthesisofsteroidalsaponins