利用活性污泥生物合成聚羥基丁酸酯的研究.pdf

1、天津大學碩士學位論文利用活性污泥生物合成聚羥基丁酸酯的研究姓名：德吉央宗申請學位級別：碩士專業(yè)：環(huán)境科學指導教師：趙林20090601ABSTRACTPoly—D—hydroxybutyrate(PHB)isanintracellularstoragesubstanceproducedbyalargevarietyofbacteriaIthasattractedinterest勰biodegradableplasticsnotonlyb

2、ecauseofitscompatiblematerialpropertieslikesyntheticthermoplasticsbutalsobecauseofitcouldbesynthesizedfromrenewableresourceThemicroorganismsinactivatedsludgewereabletoaccumulatePHBintheformofintracellularstoragepolymersT

3、heproductionofPHBfromresidualsludge，therefore，isanewwayforPHBsynthesis，whichalsodevelopsanovelpathwayforthereclamationofresidualsludgeAsequencingbatchreactorwasconductedbyaerobicdynamicfeedingwithacetateascarbonsourcetod

4、omesticateactivatedsludgetoenrichPHB—accumulatingmicroorganismsAndtheacetateremoval，PHBaccumulationandtheactivatedsludgegrowthinatypicalcycleWasalsostudiedAfterbeingdomesticated，theunbalancedgrowthofactivatedsludgeandthe

5、PHBaccumulationWasresearchedTheresultsshowedthatfeedingbyhighacetatedosageof9315mgC／L，theactivatedsludgegrewunderunbalancingconditionTheincreaseofactivatedsludgebiomassismanlyduetotheaccumulationofPHBDuringfeastperiod，94

6、％acetatewasremoved，inwhichafractionof534％ofacetateWastransformedtOPHBTheapparentincreaseofactivatedsludgewas880mg／L，inwhichtheincreaseofPHBcontentis836mg／LToimprovethePHBcontentinactivatedsludgeandtofindtheoptimalconditi

7、onforPHBsynthesizationThreecriticalfactors：dissolvedoxygencarbonconcentration，andmtrogendeficiencyWasevaluatedTheresultsshowedthatO)therateofsubstrateuptakeaswellasthecontentofPHBincreasedwiththeincreaseofdissolvedoxygen

8、concentrationbuthighDOsupplyrateresultedindecreasedPHByieldsForthemostefficientconversionofacetatetoPHB，amoderatedissolvedoxygensupplyappearstObeappropriateAtamoderatedissolvedoxygensupplyrateof80L／h(KLa8931／h)，amuchhigh

9、erconversionof076wasreache@withtheinitialacetateincreasing，themaximumPHBproductionincreasedcorrespondinglyFeedingbyahighdosageacetateof800mgC／L，withaF／M(FoodtoMicroorganisms)of25mgCLq／mgCL～，PHBproductionreacheditsmaximum