各種緩沖液的配制方法-

1、各種緩沖液的配制方法各種緩沖液的配制方法Na2HPO4檸檬酸鈉緩沖液PH0.2molLNa2HPO4(ml)0.1molL檸檬酸鈉(ml)PH0.2molLNa2HPO4(ml)0.1molL檸檬酸鈉(ml)2.20.419.65.210.729.282.41.2418.765.411.158.852.62.1817.825.611.608.402.83.1716.835.812.097.913.04.1115.896.012.637.

2、373.24.9415.066.213.226.783.45.7014.306.413.856.153.66.4413.566.614.555.453.87.1012.906.815.454.554.07.7112.297.016.473.534.28.2811.727.217.392.614.48.8211.187.418.171.834.69.3510.657.618.731.274.89.8610.147.819.150.855.

3、010.309.708.019.450.55Na2HPO4。2H2O，分子量=178.050.2molL溶液含35.61gL檸檬酸.H2O，分子量=210.140.1molL溶液含21.01gL檸檬酸檸檬酸鈉緩沖溶液（0.1molL）PH0.1molL檸檬酸（ml）0.1molL檸檬酸鈉（ml）PH0.1molL檸檬酸（ml）0.1molL檸檬酸鈉(ml)3.018.61.45.08.211.83.217.22.85.27.312.73

4、.416.04.05.46.413.63.614.95.15.65.514.53.814.06.05.84.715.34.013.16.96.03.816.24.212.37.76.22.817.24.411.48.66.42.018.04.610.39.76.61.418.64.89.210.8檸檬酸.H2O，分子量=210.140.1molL溶液含21.01gL檸檬酸鈉.2H2O，分子量=294.12；0.1molL溶液含29.4g

5、L4.817.5025.00100.005.023.6525.00100.005.229.7525.00100.005.435.2525.00100.005.639.7025.00100.005.843.1025.00100.006.045.4025.00100.006.247.0025.00100.000.2molL苯二甲酸氫鉀溶液：稱取在硫酸干燥器中干燥過24小時(shí)的苯二甲酸氫鉀20.44g溶于水中，稀釋至500ml。磷酸緩沖液（0.

6、2molL）PH0.2molLNa2HPO4(ml)0.2molLNaH2PO4(ml)PH0.2molLNa2HPO4(ml)0.2molLNaH2PO4(ml)588.092.07.061.039.06.012.387.77.272.028.06.218.581.57.481.019.06.426.573.57.687.013.06.637.562.57.891.58.56.849.051.08.094.75.3Na2HPO4.2H

7、2O分子量=178.05，0.2molL溶液含35.61gLNaH2PO4.2H2O分子量=156.03，0.2molL溶液含31.21gLNaH2PO4.H2O分子量=138.03，0.2molL溶液含27.6gLNa2HPO4.12H2O分子量=358.22，0.2molL溶液含71.64gL磷酸鹽緩沖液（PBS）配制方法0.01MPBSPBS(135mMNaCl2.7mMKCl1.5mMKH2PO48mMK2HPO4，pH7.2)

8、PBS緩沖液（pH7.2～7.4）：NaCl137mmolL，KCl2.7mmolL，Na2HPO44.3mmolL，KH2PO41.4mmolL稱7.9gNaCl，0.2gKCl，0.24gKH2PO4(1.44gNa2HPO4)和1.8gK2HPO4，溶于800ml蒸餾水中，用HCl調(diào)節(jié)溶液的pH值至7.4，最后加蒸餾水定容至1L。保存于4℃冰箱中即可。需要注意的是，通常所說的濃度0.01M指的是緩沖溶液中所有的磷酸根濃度，而非Na