水稻葉尖枯突變體wlt1的遺傳分析與基因定位.pdf

1、揚(yáng)州大學(xué)碩士學(xué)位論文水稻葉尖枯突變體wlt1的遺傳分析與基因定位姓名：朱觀林申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：作物遺傳育種指導(dǎo)教師：錢前20090501揚(yáng)州大學(xué)碩士學(xué)位論文GeneticAnalysisandGeneMappingofaWitheredLeaftipMutantinRice(OryzasativaL)Abstract2一Leafisanimportantorganforphotosynthesisandrespirationinp

2、lants，andplaysakeyroleinitslifeactivityAlsoleafdevelopmentisimportantforplant—typesandgrainproductionLeafsenescenceinriceCanleadteductionofphotosynthesispowerandgraduallossofassimilationabilityThenitaffectstheformationan

3、daccumulationoforganiccompoundsinthelaterstageofricegrowth，andtheformationofproductionSoleafsenescenceisoneofthelimitingfactorsinidentificationandutilizationofficehi曲yieldpotentialAwitheredleaftipl@／t1)mutantofrice(Oryza

4、sativaL)bymutagenesisofethylenemethylsulfonate(EMS)treatmentfromNipponbarewasidentifiedandusedinthisstudyThemutantexhibitedphenotypesofleafscorchandwitheredleaftip，andheavierleafscorchandemergenceofuppernodetillersduring

5、latergrowthstageInthisstudyweusethewltlmutanttoconductsomeresearchonphysiologiccharacters，geneticanalysisandgenemappingTheyarebeneficialtodissectionofmelocularmachenismofleafsenescenceandupper—nodetillersinriceToanalyzew

6、hetherwltlWascontrolledby。asinglegene，geneticanalysiswithreciprocalcrossesbetweenwltlandallindicacultivarsTNlorZF802wasconductedandrevealedthatwltlpossessearecessivemutationBasedontheirF2segregationindividualsderivedfrom

7、thecrosses，theratioofmutantphenotype：wildtypewas1：3髟c∥o05=384)，confirmingthatthewltlphenotypeWascontrolledbyasinglerecessivegeneAlMictestsbetweenwltlandanothermutantwlt2showedthattheyaredifferentgenesCytologyobservationo

8、fwltlinlatergrowthstageindicatedthatthevascularbundlesizeinthemutantissmallerthanthatofthewild—typeChlorophyllcontentdetectionoffunctionalleavesbetweenwltlandthewild—typeduringtilleringstageshowedthereductionofthetotalch

9、lorophyllcontentinwltlwhichmainlyresultsfromthereductionofChlaUsing142F2mutantindividualsfromthecrossbetweenwltlandTNl，the脫丌genewasprimarilymappedtotheregionofthelonga]旺nofchromosome10betweenthetwomarkersSTS1(669cM)andSS