水楊酸對(duì)CMV的誘導(dǎo)抗性及抗性累加效應(yīng)的研究.pdf

1、河南農(nóng)業(yè)大學(xué)碩士學(xué)位論文水楊酸對(duì)CMV的誘導(dǎo)抗性及抗性累加效應(yīng)的研究姓名：師金鴿申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：煙草學(xué)指導(dǎo)教師：楊鐵釗20090601TheResistancetoCMVandItsCumulativeEffectofTobaccoPlantInducedbySASupervisor：ProfYangTie出aoPostgraduate：SiftJingeABSTRACT：Toseriousharmofcucumbermosai

2、cvirus(CMV)，whiletheeffectiveprophy’lactico—therapeuticmeasureshavenotbeenfoundonagriculture；ThevarietiyofK326cultivatedareainthecountryiscomparativelylargeItsoutput，qualityetcareabettercombinationInparticulargoodquality

3、islessthantheothervarieties，butitisnotoneoftheshortcomingsofagainstmosaicdiseaseInthispaperafterCMVinoculated，thediseaseindex，diseaserelatedactivityandhydrogenperox—ide(H202)weredeterminedSalicylicacid(SA)inducetoCMVindu

4、cedresistancetonansferandcumulativeeffectwerestudiedMajorfindingsoftheresearchareasfollows：TobaccolaminaesprayedbySAandMeJA，whichallcouldinducetobaccoplanttesistCMVthediseaseindexreducedTheinducedimpactWas3358％and3224％se

5、parately；SprayedbySAandMeJA，andafterCMVinoculated，bothmeasures，couldsignificantlyincreasethetobaccoleafenzymeactivities，whichconsistofsuperoxidedismutase(SOD)，peroxidase(POD)，andincreasedthecontentofH202，lowercatalase(CA

6、T)activityThismeansthat，SAandMeJAcouldincreasedthetobaccobody’SSODactivityandPODactivityreducedCATactivitytoimprovetheH202contentoftheSARsecondmessengerstoinducetheSARinplantstoenhanceresistancetoCMvSAtodeal塒tlltobaccoon

7、behalfoftheG1，thediseaseindexdecreasedsignificantly口001)lowerthanantitheses，G1effectofgenetictransmissionreachto3328％，andhaveincreasedtheactivitiesofPOD，polyphenoloxidase(PPO)andphenylalanineammonialyase(PAL)insidethetob

8、accoplantsCumulativeeffectintheresistancetest，AftertheG1andG2wereinducedbySAgotvaccinatedCMVthediseaseindexcompared而t11theantitheses，weresignificantlylowerthecumulativeeffectsrespectivelyachieve3581％and4470％，andthediffer

9、encebetweenthetwosignificantly；Atthesametime，intobaccoplantofG1andG2，thePOD，PPOandPALactivitiesarehigherthantheantithesesInshort，SAandMeJACaninducetobaccoplanttesisttoCMVDealedwithSAnotonlythetobaccoplantinducedagainstCM