中國原產(chǎn)完全甜柿‘小果甜柿’DkMYBa基因的克隆和分析.pdf

1、華中農(nóng)業(yè)大學碩士學位論文中國原產(chǎn)完全甜柿‘小果甜柿’DkMYBa基因的克隆和分析姓名：江麗萍申請學位級別：碩士專業(yè)：果樹學指導教師：羅正榮20090601ABSTRACTTodate。thestudiesoftheidentificationofthepropertyofnaturalLossofastringencyonPCNA(Pollinationconstantnonastringent)inchinashowtheastrin

2、gencylossprop。ertyofthenativechinesePCNAtypesisdifferentfromJapanesePCNAtypes，becauSe也ere鰣ationofthetanninbiosynthesissynthesizemechanisminchinesedeastringentedperSim咖onmaybedifferentfromtheJapanesepersimmon，butitsdetail

3、sarenotyetclearInthisstudy，weuse’Xiaoguotianshi’(DiospyroskakiThunb)asthetestmaterial，clomngtheeDNAsequenceofMYBtranscriptionfactorgenesucessfully，andwewillpredictthefunctionandanalysisitsexpressionThemainexperimentalres

4、ultsareasfollows：1SequencebyhomologuscloninghasbeenthepersimmonMYBtranscriptionfactorgeneAftersequencealignmentsofaminoacidtranslatedwithotherplants’sequenceitalsohasthetypicalcharacteristicswhichissameasMYBgeneasinother

5、plants：withthe卿icalR2R3regionwhichCanformahelixloop。helixstructureregarding2LsamaioritytagofMYBtranscriptionfactorgenes，bHLHbindingsite，t、】~，omorethanL—tryptophangroupfollowedbyanumberofconservativeaminoacidsSequenceali窖

6、fmmentsalsoshowahigheridentitybetweenMYBgeneinpersimmonandthemInotherpl齜s，whichisupto85％IdentityofaminoacidsequencesofDkMYBageneishighestinallaligmentedsequence，whichisbetweeninpersimmonandincotton2TheresuItsofaprelimina

7、ryexpressionanalysisofDkMYBatranscriptionfactorgenesin。Xiaoguotianshi’sfruitshowedthattheDkMYBageneisexpressingfromMaytomidJulyinpersimmon，afterAugust11thegenieexpressionstoppedlneexpressiontrendlineofDkMYBageneiscoincid