基于T-RFLP技術的肉雞消化道微生物群落多樣性的研究.pdf

1、中國農(nóng)業(yè)科學院碩士學位論文基于TRFLP技術的肉雞消化道微生物群落多樣性的研究姓名：張華申請學位級別：碩士專業(yè)：動物營養(yǎng)與飲料科學指導教師：蔡輝益201106AbstractInthisdissertationmicrobialdiversityanditsd)r11amicchangesinbroilercaecumwerestudiedusingtechniqueofterminalrestrictionfragmentlength

2、polymorphism(TRFLP)，andinfluencesofdietarychlortetracyclineandcellulose011growthofmicrobialcommunityinterminalileumofbroilerwerefurtherinvestigatedExperiment1TRFLPmethodonmicrobialcommunitydiversityanalysisinbroilergastr

3、ointestinaltractTRFLPmethodofstudyingthediversityofbroilergastrointestinalmicrobialcommunitywasestablishedbyoptimizingPCRconditions(selectionofprimeramountoftemplate，thereactionsystemannealingtemperature，andothers)accord

4、ingtocharacteristicsofmicrobial16SrDNAinbroilergastrointestinaltractRestrictionenzymedigestiontimewasalsoexploredPeal【ScannersoftwareandTRELPnetworkdatabasewereappliedinthemethodExperiment2Studyofdiversityanddynamicchang

5、姻ofmicrobialcommunityinbroilercecaTRFIPtechniqueWasusedinthistrialtostudythediversityanddyIlamicchangesofmicrobialcommunityinbroilercecatoexplorethemechanismhownutritionrelatedtogrowthanddevelopmentofgastrointestinalmicr

6、obialcommunityTheresultsshowedthatthececalmicrobialcommunitydiversityWasdifferentatdifferentages，butthedifferencewasnotsignificant(PO05)Asbirdsgrewtherichnessofcecalmicrobialcommunityspeciesdecreased，andthedominantspecie

7、sbecamemoreobviousIntherelativecontentofrepresentativeTRFsismorethan05％oftotaltheflorasofTRF36，Tl訌B7，TItF39，TRF57，TRFl64，TRFl68，TRFl88werenormalmicroflora，whiletheflorasofTRF52。TRFl86，TR脒weretemporaryfloraTI礎techniqueapp

8、liedinthistrialisagoodtooltoanalyzethediversityanddyIlamicchangesofmicrobialcommunityinbroilergutExperiment3EffectsofchlortetracyclineonmicrobialcommunitydiversityinbroilerterminalileumThistrialwasconductedtostudytheeffe

9、ctsofchlortetracyclineonmicrobialcommunitydiversityinbroilerterminalileumduringthestarterperiodaswellastoexplorethemicrobialdetectionmethodofchlortetracyclineTheresultsshowedthatduringthestarterperiodofbroilertheeffectso

10、fchlortetracyclineonmicrobialcommunitydiversityintheterminalileumwereexistManyTRFswereobtainedinthisexperimentandtherewere17TRFswhichwererepresentativeandtheirrelativecontentismorethan05％oftotalOftheseTI己Fs，TRF28。T砌P2l6。

11、TI己F596wereinhibited，whileTRF48，T剮昭7，TRF88，TRF95，TRFI68，TRF255werepromotedTheinfluenceofehlortetracyclineonunculturedmicroorganismsintheterminalileumcallbedetectedMoreimportantlythemicroorganisms(TRF596andTRF95)thatweres

12、ensitivetochlortetra_cyclineCanbedetectedaswellItCanbeconcludedthattwoTRFsmaybetheobjectivefragmentusedinthemicrobialdetectionofchlortetracyclineExperiment4EffectsofcelluloseOHmicrobialcommunitydiversityinbroilerterminal