中華絨螯蟹血清凝集素生物學(xué)特性的研究.pdf

1、安徽農(nóng)業(yè)大學(xué)碩士學(xué)位論文中華絨螯蟹血清凝集素生物學(xué)特性的研究姓名：劉雪蘭申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：預(yù)防獸醫(yī)學(xué)指導(dǎo)教師：余為一20020101StudiesonBiologicalPropertiesoftheLectininSerumofEriocheirSinensisAbstractMethodsofmicroagglutinationassayorthogonalexpetimemaldesign，SDSPAGE，dotELISAw

2、ereusedforbiologicalcharacterizationoflectininserluTlofrivercrab，EriocheirsinensisAmicroagglutinationassaywascarriedoutonserumlectinactivityofcrabTheresultsofshowedtllattheserumcouldagglutinateerythrocytesofchickenrabbit

3、，culvermouse，human(A，B，Otype)，butnottheseofcmcianandturtleItcouldalsoagglutinatebacteriasuchasSarcinaandAeromonashydrophila(J1)excludingEcoli，parvobacteria，Staphylococcusaureus，VibriomimicusH7andPseudomonas12Moreover’asi

4、gnificantl)’NghertiterwasobservedinthemicroagglutinationassaybetweencrabserumandmousecellsorSarcinathanandotherkindsofanimalerythrocytesorbacteria(pO05)Presenceofaconcentrationwith001MCa2or、vitllO3MNaCIcouldnotinfluencet

5、heactivityofagglutinatingmousecellincrabserum，butwithO6MNaCIthehemagglutinationtitercouldbedecreasedTheserumtreatedbyO104MEDTAatpH50lostitshemagglutinatingactivi班A33designoforthogonalexperimentwasusedtodetecttheeffectoft

6、hreekindsoffactorsontheagglutinatingactivityofthecrabserumTheresultshowedthattheactivitydidn’tchangewhenwatertemperaturewasaltered(pO05)andexpressednodifferencebetweengroupswithdifferentweightofcrabsHoweverinjectionofSar

7、cinaorsalinecouldinducecrabsignificantlytoincreasethetiterofagglutinatingbacteria(p005)12dafterinjectingtheagglutinationtiterwasincreasedhi曲lyandthenrestoredtonormallevelupto12d14dInaddition，repeatedinjectionsotbacteriae

8、very5d7dwouldkeeptiterlevelofagglutinatingbacteriaErythrocytemembraneofmousewasusedastheaffinitymatrixforisolationoflectinfromcrabserumThetreatedlectinhadagglutinatingactivityagainstrnouseerythrocyteandshowedthreebandsab