29362.海帶硫酸多糖的提取純化降解及活性研究

1、曲阜師范大學(xué)碩士學(xué)位論文海帶硫酸多糖的提取純化降解及活性研究姓名：程斌申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：植物學(xué)指導(dǎo)教師：趙云峰20090401曲申師范大學(xué)2006級(jí)碩士畢業(yè)論文AbstractLaminariapolysaccharidesarethemainactivitysubstanceinLaminariakelp，suchasalgin，fucoidanandlaminaranFucoidan，theacidpolysaccharide

2、s、Vithsulfateesterhavemanybiologicalpropertiesandpharmacologicalfunctions，suchasimmunityregulationantitumorantigrume，loweradipose，lowersugarradioprotection，antimutationandanti—oxidationThispaperdiscussedthemeritsofLPSext

3、ractionandfoundaneconomicalandsimplemethodofpurificationofpolysaccharidesBydeterminingthebestconditionsofUltrasonicdegradationofLPSandanalysisofpolysaccharidedegradationactivitytwoOligosaccharideswithhigheractivitywereob

4、tainedThepolysaccharidesulfatewasextractedfromLaminariajaponicabywaterextractionandenzymeextraction，then，theextractionrateWascomparedThecrudeLPSwerepurifiedelementarily，throughextractedbyCTABanddecolorizedbymacroporousre

5、sinTheoptimumconditionsofpurificationwereconductedTheeffectsofpowerofultrasonic，concentrationofLaminariapolysaccharidesulfate，ultrasonicprocessingtimeonthedegradationofLaminariapolysaccharidesulfatewerestudiedbyultrasoni

6、cdegradationThroughsinglefactorsandorthogonalexperiments，theoptimumconditionsofultrasonicdegradationwereconductedJointedwithultrasonic，H202wasusedtodegradethepolysaccharidesulfate，andtheresultWascomparedtothatofH202After

7、degradation，theproductwasgradedwithethan01TheantioxidanteffectsofL3，L5werestudiedfromthreedifferentexperiments：eliminatingsuperoxideanionfreeradicalexperiment、inhibitingautoandinducedlipidperoxidationinrateliverhomogenat

8、eMTTcellviabilityassayandHoechst33258stainingexperimentswereconductedtocomparethegrowthinhibitoryactivityontumorcellA549TheresultshowedthatenzymeextractingmethodWasmoreeffectivethanthatofwaterextraction，theextractingrate

9、ofcrudeLPSandpureLPSwereincreasedby859％and436％respectivelycomparedwiththatofwaterextractionThecrudeLPSwereprecipitatedusingCTABasprecipitantThroughorthogonalexperiments，weobtainedtheoptimaltechnologicalparameter：ratioof1

10、％LPSand5％CTAB3：2，precipitationtime6h，centrifugaltime12minDecolorizingexperimentswerecardedoutbysingleexperimentThebestconditionsfordiscolorationweredeterminedasfollows：D315resinratioof1％LPSandresinWas100：1(V／W)，Time=6h，p

11、H=50，T=40。CTheoptimumconditionswereasfollows：powerofultrasonic200WconcentrationofLaminariasulfate49／L，ultrasonicprocessingtime15minUndertheconditions，thedegradationcharacteristicviscosityWas0987mL／g，molecularweightwas464