化學(xué)雜交劑（CHA）誘導(dǎo)小麥雄性不育的可能機(jī)理.pdf

1、河北師范大學(xué)碩士學(xué)位論文化學(xué)雜交劑（CHA）誘導(dǎo)小麥雄性不育的可能機(jī)理姓名：李則軒申請(qǐng)學(xué)位級(jí)別：碩士專(zhuān)業(yè)：細(xì)胞生物學(xué)指導(dǎo)教師：黃占景張愛(ài)民20030601ABSTRACTThisworkfoCHSonisozymeanalysisandcytologicalstudiesontheeffectoftwochemicalhybridizingagentsBAU9403andGenesis，bothcouldinducemalesteril

2、ityinwheatsuccessfullyInthecourseofpollenabortion，thechangesofperoxidase(POD)andesterase(EST)wereassayedTheresearchresultshowed：(1)thetendencyofperoxidasechangesinducedbytwoCHASweresimilarThePODactivityinantheroftreatmen

3、tswas10werthancontr01whichindicatedthatwheatmalesterilityinducedbyCHAwascorrelatedwiththedecreaseofPoDactivity(2)therewereobviousdifferentisozymebandsbetweenBAU9403andGenesisinducingmalesterilitywhichindicatedthatdifiere

4、ntCHAShaddifierentmechanismtOnducemalesterilityThezymogramsofPODshowedthatwhentreatedbyGenesis，fromthe2。”daytheexpressionofPODbegantodecrease，butforwheatusedwithBAU9403fromthel“dayactivityofisozymedecreased1’wokindsofCHA

5、jnfluenceddifferentisozvmebandsr3、thetwoCHAshadnotevidenteffectonESTactivitywhichshowedthatdifierentisozymeshaddi行erentresponsetoCHAAtthesametimewedesignedanotherexperimentaddingCHAinvitroWeaddedGenesisintoenzymeliquiddi

6、rectlyandfoundthattheCHACaninhibitPOD’SactivitybuthadlittleinfluenceonEST’SactivityThisnewdiscoveryhelpUStounderstandfurtherthemechanismofmalesterilityinducedbyCHAEmployingopticalandelectronicmicroscope，themorphologicala

7、ndcytologicalfeatureofwheatantherinducedbyCHA～BAU9403andGenesisfordifferentdosagesThemorphologicalfeaturewasasfollow：thesterileplantwaslowandlightgreenincolorThespikeexpandedandtheantherwasnotopenThecytologicalstudiessho

8、wed：(1)TheabortivedegreeofthepollenfromBAU9403inducedmalesterilewheatWasrelatedcloselywithCHA’SdosageThereexisthighersteriledegreeinplantunderhighdosagethanunderlowdosage(2)Thefunctionalabnormalityoftapetumandvascularjna

9、ntherinducedbyCHAwasresponsibleformalesterility(3)Aboaiveprocessbeganfromtetradstage，andcontinueuntiltotheendofthinucleatestageAlthoughabortionoccurredatalldevelopmentaIstages，pollenabortivefeatureWasmostobviouslyduringu

10、ninucleatestage(4)TheabortiveindicationwasthattapetumdegeneratedabnormallythedevelopmentofvascularWasbad，cell—organellesdisorganizedearlierthannormalpollen，pollenwallwasthinnerandnotarrayedcompactlypollenWaswizenedandthe