28312.擬南芥轉(zhuǎn)錄因子tdf1在花藥發(fā)育過(guò)程中分子調(diào)控機(jī)理的研究

1、上海師范大學(xué)碩士學(xué)位論文擬南芥轉(zhuǎn)錄因子TDF1在花藥發(fā)育過(guò)程中分子調(diào)控機(jī)理的研究姓名：周茜申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：生物化學(xué)與分子生物學(xué)指導(dǎo)教師：楊仲南20100301I：泡師范人學(xué)碩l：學(xué)位論文ABSTRACTInArabidopsis，mF，encodesaMYBtranscriptionf2lctorWhichplaysaVitalroleint印etaldeVelopmentand如nctiond嘶ngaIltherdeVelop

2、ment刪washi曲lyexpressedinmeiocytes，t印etumandmicrosporesinantherdeVelopmentThetdflmutantsho、vsacompletemalestemephenot)，peIIlthisstudy，the到竹Ygene、vasobtainedbyPCRmethodandclonedintotheexpressionVectorpET32aARertheprol卿otic

3、expressionVector‘TDFl一pET32a’introducedintoE∞，fBL21(DE3)，theTDF1recombinantproteinof562KD、VasexpressedbyinductionwithIPTGThe如sionproteinm旬orlyeXistediIltheinclusionbodyARerex位屺tedf硒mt11einclusionbody，theTDF1proteinwasuse

4、dastheantigentoimmunerabbitsELISAassayshoWedthatthetiterofthepreparedpolyclonaJ鋤tibodywaS1：2500Ttle，immunologicalspecifici鑼ofthepolyclonal觚tibodytoTDFl、糯coIlfi肌edbyWestemblotanalysisThepreparedantibodyin“sworkshould矗Kili

5、ta_teme缸ther向nctio眥linvestigationof功F，iIlbiochemicalleVelsin彳陽(yáng)6坳西antherdevelopmentBioi—’onnaticsanalysisidentifiedseVeraltmscriptionfactorsmaybeinvolvedintheregulationof7DF，F(xiàn)urthermore，metransgeniclineofTDF1andTAP—tagmsi

6、onproteinwaSobtained，whichproVideeaSyaccesstofindthedirectta唱etofTDF1byChIP11leseworkslaythefoundationforfhrtherinVestigationofthemolecularbasisofTDF1ina11therdevelopmentKeywords：Arabidopsis；T№scriptionfactor；Fusionprote