45290.桑黃水溶性多糖的分離純化及結(jié)構(gòu)研究

1、學(xué)校代碼：!妲塑分類號：Q5④Y888206研究生學(xué)號；1212QiQ絲密級：孟東弘嶧葒大季碩士學(xué)位論文桑’黃水溶性多糖的分離純化及結(jié)構(gòu)研究ThepurificationandstructureinVestigationofPheinusigniariuWatersolublepolysaccharide作者：姜德志指導(dǎo)教師：學(xué)科專業(yè)：研究方向：學(xué)位類型：張麗萍教授生物億學(xué)與分子生物學(xué)糖類生物化學(xué)學(xué)歷頑士東北師范大學(xué)學(xué)位評定委員會200

2、6年5月AbstractTheaimofthepresentresearchwaStodetecte也efbmentationtechnologyofmeⅣ擁淞f糾f甜’mmycelium，isolatemep01ysaccharidesoffenTlentalproductandpresumethepolysaccharides’s協(xié)lcnlreTbdetectthe南mlenta￡iontechnoiogy，thecontentof

3、saccl謝de，protein，01igosaccharideaIld協(xié)lceelement，t11ecompositionofmoIlosaccharide，aminoacid叭d仃aceeIementa11dthemolecularweigbtdi謝butionoffenTlentalproductiIldi舭rentlotsWeredetenninedTheresultsdemons訂讎edthatt11ecomposition

4、andcontentofdi虢礎(chǔ)呲lotsweresiIllilarand血etechnology、vasstableThefocusofthisresearchwastostudymepolysaccharidesofm出門淞劫脅rmmyceiiumAcmdep01ysacdlaridew髂ex缸acted丘_omtllefe珊etalpowderofP柳抽rf螂byboilingwateLnscontentofsaccharidea

5、ndproteillwastestedbyphenol—stllphuricacidandKjeldahldetenll血ationnscompositionofmonosacch撕de、Ⅳasdete玎ninedbygasclromatoFaphyAfcerp嘶fiedbydeproteinization誦血eTlzymea11dSeVagmethodandfactionatedby丹eezetllawing，polysacchari

6、dePIPwaSobtainedPIP2waSpreparated療omPIPusingSepharoseCL一6Bpr印盯砒ioncolurnnAf【erdemonstratedPIP2’shomogenicitywithhighperfonllance1iquidc1romatogmphy，itss虹ucturewasanalysisbypartialacdhydroiysis，Periodateoxidatioll，Srlli山d

7、egmdati011mandMemylationThcdataobtainedi砌catedthatPIP2wasaneuⅫhomoglycanwhichmolecuIarWeightwas18，000Dand91ucosidcbondswerepconfigIlrationnhadfewbranchOnaverage，therewere15brancheseve珂lOhexoseresiduesPIP2possessedabackbo

8、neof(1_4)1ir止edglucoseresidues，withsubstitutionat0—3by(1_3)一linkedgIucoseThesidechajnswascomposedof(1—3)一l址edglucose、vitlldi胝ntdegreeofpolyTnerizationnleendwascomDosedofGlcKeywords：Phelli肌si鰣ariu，ex廿atingandpurification，