25569.基于arm的酶鏈聚合擴增儀溫度控制研究

1、浙江大學機械工程學系碩士學位論文基于ARM的酶鏈聚合擴增儀溫度控制研究姓名：陳世澤申請學位級別：碩士專業(yè)：機械電子工程指導教師：陳章位20100101浙江大學碩士學位論文AbstractAbstractPolymeraseChainReaction，whichiScalledPCRforshort，isakindofnucleicacidamplificationtechnologyinvitroUsingthistechnique，r

2、easearcherscanproducemillionsofcopiesofspecificsequenceswhichareselectedfromcomplexDNAmoleculesThePCRinstrumentistheinstrumentwhichCanfinishthepolymerasechainreactionautomaticallyAtpresent，PCRtechnologyhasbeenwidelyappli

3、edinlifescienceresearch，biochemicalanalysis，clinicaldiagnosis，druganalysis，forensicidentificationandepidemicrapidtestetcThisdissertationdiscussesaboutthetemperaturecontrolofPCRinstrumentFinishthehardwaredesignofPCRinstru

4、mentbasedonARM9embeddedsystemResearchontemperaturecontrolalgorithmandthetemperatureuniformitycontrolforPCRinstrumentThemaincontentsareasfollows：ThefirstchaptersummarizestheprincipleofPCRinstrument，thegenetalsituationofPC

5、RinstrumentandtheresearchsituationahoutthetemperaturecontrollerandtemperaturecontrolalgorithmhomeandabroadInthispart，Ifinallyputforwardresearchcontents，methodsanddifficultiesThesecondchapterintroducesthehardwaresystemdes

6、ignofPCRinstrumentIintroducetheconstituentsofhadwaresystemfirstandthenintroducethedesignofheatingandcoolingsystembasedonTECandtemperaturecontrollersystembasedonembeddedsystemindetailFinallyIintroducesomeassistantdepartme

7、ntslikeheatcoverbrieflyInchapterthree，IstudyabouttemperaturecontrolalgorithmAfterintroducingfeedforwardcontrolandfuzzycontrolwhicharenotdependentonmathematicmodel，Iputforwardamixturecontrolalgrithemwhichisconstitudedbyfe

8、ed—fordwardcontrol，fuzzycontrolandrelayauto—turningPIDcontr01ThefourthchapterresearchonthePCRuniformtemperaturecontrol，establishedthefiniteelementsimulationmodelofthesampleblock，gotitstemperaturefield，improvedthesamplebl

9、ockmodelFromtheresultofthesimulationandexperimentswecanfindoutthatthisimprovedmodelcalleffectivelyimprovePCRtemperatureuniformityInthechapterfive，IdosomeexperimentsonthePCRprototypewiththerelayautotumningPIDalgorithm，fee