假大白菇、皺柄白馬鞍菌生物學(xué)特性與營養(yǎng)成分的研究.pdf

1、西北農(nóng)林科技大學(xué)碩士學(xué)位論文假大白菇、皺柄白馬鞍菌生物學(xué)特性與營養(yǎng)成分的研究姓名：王俊明申請學(xué)位級別：碩士專業(yè)：植物學(xué)指導(dǎo)教師：胡景江曹支敏20040601AbstractRussulapseudodelicaLangeisakindofedibleandmedicinalmushroomItsstromaisrecordedas‘jiadabaigu”inChirlaItisaniceediblemushroomandcontalne

2、danticancersubstance，sotheforegroundofexploitureonRussulapseudodelicaLangeisfascinatingThereisstillnotrespectofbiologicalcharacteristicandnourishmentcomponentaboutRussulapseudodelicaLangeInourstudythenitritiveCharacteris

3、tics，physiologyofsubmergedcultureoptimalconditionsforsubmergedfermentationcultureand也enutritioncomponentsofRussulapseudodelicaLangewereexplored，Andstill，thephysiologyofsubmergedcultureandthenutritioncomponentsofanotherki

4、ndofediblefungusHelvellacrispa(sceD)Frwerestudiedrr∞r(nóng)esultisasfollowsThenutritivecharacteristicsofRussulapseudodelicaLangewerestudiedTheresultsshowedthatthetemperatureandpHappropriatetoitsmycelialgrowthwere10—30“Cand5011

5、0rasp：thatthebestcarbonsourceswassucrose；theoptimalnitrogensourceswereKN03andbeefextract；anddifferentvitaminsaddedhadnosignificanteffectonitsmycelialgrowthForthefirsttimedynamicvariationof5physiologicalindexand6kindsofex

6、tracellularenzymeduringthesubmergedcultureofRussulapseudodelicaLangewereinvestigatedTheresultsshowedthatthemaximumofhyphagrowthwas6589／Lappearedinthe10“day；pHoffluidmediumrisesafterlowering；thepeaktimesofextracellularpro

7、teinanddeoxioizedsaccharidewerethe5“daythe6“day；extracellularpolysaccharideappeareditsmaximuminthe6”day；dynamicvariationofdiastase，CMCenzyme，cellulaseduringthesubmergedculturewerequitesimilar；theactivityofguaiacoloxidase

8、andpolyphenoloxidasewerevery10wduringthesubmergedcultureTheresultsofoptimalconditionsforsubmergedfermentationcultureshowedthatthebestcarbonsourcesofthesubmergedcultureofRolSSbllapseudodelicaLangewassucrose；theexcellentni

9、trogensourcewasKN03；theappropriatepHwas60；thesuitabletemperaturewas25℃：theappropriateinoculatequantitywaslO％；thebestcombinationofvolumeandshakefrequencywasvolume75mlunder廿1eshakeof100r／min；theexcellentcombinationofcarbon

10、sourceandnitrogensourceweresucrose49／100m1KN030059／100ml；Eightextracellularellz3rmesandfourphysiologicalindexwerestudiedduringthesubmergedculture磺HeIvellacrispa(Scep)Frforthefirsttime。Theresultsshowedthatthemaximumofhyph

11、agrowthappearedinthe5”day；pHoffluidmediumrisenafterappearedtheminimumin血e2noday；也epeaktimeofextracellularproteinappearedinthe6“day；afterreachthemaximuminthe2“odaytheactivitvofextracellulardeoxioizedsaccharidedroppedandba

12、lantedafterthe9thday；恤eactivityofCMCenzymeandcelluloseofcottonwoolwerequitesimilar；thepeaktimeofFPaseappearedinthe6。dayand8”day；activityofamylaseappeareditsmaximuminthe4“day恤endroppedandtheenzymeactivitystillkeptahighlev

13、el；inthe3”dayofsubmergedcultureactivityofacidinvertasereacheditsmaximum；theactivityofneutralinvertaseincreasedlinearlyasthetimechanged：aetivityofgualacoloxidaseandpolyphenoloxidasewereverylowduringthesubmergedcultureThet