我國蠶豆根瘤菌的多相分類與系統(tǒng)發(fā)育研究.pdf

1、中國農(nóng)業(yè)科學院碩士學位論文我國蠶豆根瘤菌的多相分類與系統(tǒng)發(fā)育研究姓名：路敏琦申請學位級別：碩士專業(yè)：微生物學指導教師：李俊20050601AbstractRhizobiaisolatedfiombmadbeanin24differentregionsof11pmxincesofChinawe]i“eanalyzedbynumericaltax衄omvBOX—PC＆16SrDNAPCRRFLPandIGSPCRRFLP16SrDNAfuU

2、lengthsequencingandcrossnodulafion∞幣erimentsTheanalysisofBOXpCRdividedthe73swai／1sinto18groupsand5unattachedbranchat血esimilarityof70％：Basedon也eresultofBOXPCR，丘腫SlIainsⅥ‘th11referneneeslyainsweretestedbynumericalanalysiso

3、f138ph)siologicalandbiochemicalcharactersTheresultshowedthatthe50sIRRilIsweredivldedinl04distinctiveclustersat也esimilarityof81％麗也∞o也er8unattachedbranch：Furthermore25sm疽nsastherepresentationof也ebroadbeanrhizobia聃‘th11refe

4、rencestrai／1swereselectedtoPCRRFlPof】6SrDNAanalysisThe25s姐i／1sⅥrereclusteredinto4groupsatthesimilarityof85％withanother1unattachedembranchment111isresultwasingood89reementwiththatofnumericaltaxonomy；Thecharacterizatlonof3

5、5strainsincluding11referenceSlR“dillSwereperformedby16S一23Sd礬Aintergenicgenespacer(IGS)RFI衛(wèi)Comparedwith16SrDNAPCRRFLPtheresultofIGSRnJshowcdmoredifferencesamongstrainsThefilllI曲山of16SrDNAofthecentersUains：XzSX0l一6、SCHYl、

6、SXYXI1andano血el“tworepresentativestrains：SCYA3SXjYXl5weresequencedandanalyzedwi也thefifteenreferencestrainsTheresultsdisplayedthatalltherepresentativestrainsbelongedtothe$alflephylogeneficbranchofRhizobiumNinteenstrainsof

7、bmadbeanrhizobiawerecomparedfortheirgrowthorsunivalabilitiesunder也eslaessesofNaCI，acidlalkali，hightemperatureandlowtemperatureTheresultsshowcdthat也ereweresomestains謝thsupcriortolerancetoNaCl，acidandalkali，whiletheirendur

8、ance曲ilifiesdifferredgreatlyTheslTainXZSX016waschosentoperformcrossnodulafionexp豇mlent$謝也6legumeplants，Theresultsshowedlbat也erhizobiumfrom也ebroadbeancanonlynodalatewinlbroadbeanandpeaKeywords：Rhizobiasp(viciafabaL)numeri