人膽管癌組織原發(fā)灶和轉移灶裸鼠種植模型的建立及觀察.pdf

1、分類號UDC：Y941937密級：請注明密級及保密期限石河子大學碩士學位論文人膽管癌組織原發(fā)灶和轉移灶裸鼠種植模型的建立及觀察研究生指導教師申請學位門類級別學科、專業(yè)名稱研究方向所在學院劉斌馮德元教授中國新疆石河子2006年6月Establishmentofanimalmodelinnudemicebyretransplantationhumaneholangiocarcinomatissueintosubcutaneousandobs

2、ervationPostgraduatestudent：LiuBinSupervisor：ProFengDeyuanAbstractOBJECTION：ToestablishaxcnografiedmodelofhilarcholgangiocarcinominnudemiceMETHOD：A丹eshhumanhilarcholangioeareinomaspecimenwasobtainedfromapatientunderasept

3、icconditionduringoperationincludedprimaryandmetastatictissueUsingpartsofprimarytissueodivideditintoinner，middleouterthreeparts，quicklycuttingtheseintolmm’smallpieces，withmnastafictiSSUeandrandomlytoestablishprimary(mer)，

4、primary(middle)，primary(outer)andmetastaticfourgroupsanimalmodels，andwillietransplantatiandobservefromgenerationtonextgeneration(total3generations)；ThetnnlorspecimenWascutintopieces，culturedin1640medium，andsubcutaneously

5、lxansplantedintoBulb／cnu／unmiceWhenthetmnorsreached10nllnindiameter，orthehostshowedcachexia，thetransplantedtumorswereretransplantedintonewmiceThemice、vereobservedevery5daysandthegrowthOfl：lImorwasrecordedandgrowthcmveswe

6、refiguredThemiceweresacrificedwhentheyshowedseriousillness，andlaparotomizedtoexminemetustatictunlorsinliverandlungsDhectdissemin面onmethodWasusedtoaffirmthekaryotype；vascularendothelialgrowthfactorfvEGF)，basicfibroblastgr

7、owthfactor(FGF2)，proliferatingcellnuclearantigen口CNA)WasexaminedⅥTitllimmunohistochemicalmethodsFlowcytometrywasusedtoexamineeveryperiodSpercentageofcellcycle：Carbohydrateantigen242(CA242)，carcincembryonicantigen(CEA)Wer

8、eexaminedinradioimmunoassay，RESULT：Thettanorwasmobile，aliflestifandsecretedamountsofmucusHEstainingshowedapoorlydifferentiatedadenocarcinomas仇lc帆，justlikethehumanhilarcholangiocareinomaspecimenThetumorWassuccessfullypass

9、aged3timesTheincnbationperiodWasshortenedtb34daysandthetmnorgrewfasterthanthefirstpassageNoliverorlungmetastatictunlorswereobservedinallthemiceVEGFFGF2，PCNAwereheavilyexpressedFloweytometryexaminationshowsthattheratioofS

10、phasefractioninmetastatictissueishigherthanthatinprimarytissueAccordingtoradioimmunoassay，thecellularconcentrationsofsometumormarkerswereasfollow：thetwomarkerswereallnegativeinSenlmExceptthefirstpassagegroachcnrve，otherp