金葡液以纖維蛋白膠為載體修復(fù)大鼠骨缺損的實驗研究.pdf

1、山西醫(yī)科大學(xué)碩士學(xué)位論文金葡液以纖維蛋白膠為載體修復(fù)大鼠骨缺損的實驗研究姓名：余建平申請學(xué)位級別：碩士專業(yè)：骨科學(xué)指導(dǎo)教師：蘇云星20080530山西醫(yī)科大學(xué)碩士學(xué)位論文ExperimentalstudyofstaphylococcusaureusinjectionrepairingskulldefectsinratswithfibrinsealantascarrierAbstractObjective：Tostudythefeasib

2、ilityoffibrinsealantascarrierofstaphylococcusaureusinjectionforrepairingbonedefects，andavoidtheflawsoflocalinjectionofstaphylococcusaureusinjectionMethods：Topreparationthecomplexoffibrinsealantandstaphylococcusaureusinje

3、ctionEiglltyWisterratswererandomlydividedintofourgroups：experimentalgroup(n=20)，controlgroupA(n=20)，controlgroupB(n20)andblankgroup(n=20)Two5mmdiameterbonedefectswerecreatedsymmetricallyonbothsidesofthecranialboneinratsR

4、arswereimplantedwiththecomplexoffibrinsealantandstaphylococcusaureusinjectioninbilateralcalvarialbonedefectinexperimentalgroup；withfibrinsealantincontrolgroupA；withstaphylococcusaureusinjectionincontrolgroupBafteroperati

5、onfor1week(containingthesameamountofstaphylococcusaureusinjectionastheexperimentalgroup)；whilenothingwasimplantedinblankgroup；AnotherfiveratswithoutanytreatmentwereregardedasnormalskullgroupAfteroperationfor4weeks，genera

6、lobservation，radiologyobservationwereperformed；afteroperationfor6weeks，generalobservation，radiologyobservation，histologicalexaminationwereperformed，biomechanicsweredetermined，andcomparedwithnormalskullResults：Afteroperat

7、ioneachgrouphadnosignificantimmunerejectionAfteroperationfor4and6weeks，radiologyobservationindicatedthatpercentageofnewboneformingareainexperimentalgroupWashigherthanincontrolgroupA，Bandblankgroup，andthereWasstatisticall

8、ysignificantdifferencebetweenfourgroups(POo1)6weekslaterhistologicalexaminationrevealedthatfibrinsealanthadbeencompletelyabsorbedinexperimentalgroupandbonedefectbasicallyhealed，butincontrolgroupA，fibrinsealanthadbeencomp

9、letelydegradedandtherewerealargenumberofnewboneattheedgeofdefect，andasmallamountofnewbonecouldbeseenattheedgeofdefectinblankgroupandotherpartsofdefecthadbeencoveredbythedensefibrousconnectivetisssue，andtheamountofosteoty

10、lusincontrolgroupBliedinbetweenitincontrolgroup1anditinblankgroup；BiomechanicsrevealedthatmaximumloadofosteotylusinexperimentalgroupweresignificantlyhighterthanincontrolgroupA&B，andlowerthannormalskullgroup(尸O05)；Maximum