旋毛蟲排泄-分泌（ES）抗原致鼠胸腺淋巴細(xì)胞凋亡的研究.pdf

1、東北農(nóng)業(yè)大學(xué)碩士學(xué)位論文旋毛蟲排泄分泌（ES）抗原致鼠胸腺淋巴細(xì)胞凋亡的研究姓名：花麗茹申請學(xué)位級別：碩士專業(yè)：預(yù)防獸醫(yī)學(xué)指導(dǎo)教師：宋銘忻20060601StudiesOilTrichinelllaSpiralisMuscleLarveESAntigen—inducedApoptosisinMiceThymusLymphocytesAbstractTrichinosisisakindofseriouszoonosisintheworld

2、whichiscausedbyTrichindiaspiralisZspiralismusclelarvaeExcretorySecretoryantigenproducedduringthegrowthstageisagoodantigenfordetectionandimmunityofTrichinosisTherecentstudyindicatedthatapoptosisinducedbyparasiticinfection

3、plays∞importantroleinmechanismofdiseaseApoptosisisneededinparasitesandrelatedwithgrowth，development，infectionandturnoverofparasitesThereisrareinformationof抒ichinosisandapoptosisinternalandexternalInordertostudytherelatio

4、nshipbetweenapoptosisandinfectionofZspiralismicethymuslymphocytesculturedinvitrowasused衢experimentmaterialsinthisstudyinducedbyZspiralismusclelarvaeESantigenwithsomeitems，suchastheactivityofcells，cellcycleandmorphologyan

5、dbiochemistryofapoptosis，detectedTheresultsshowedasfollows：1TheICs0ofESantigenonmicethymuslymphoeyteswasdetectedbytrypanbluemethodandMTTmethodrespectivelyThatis，24964046pg／mlof6h，17364068Itg／mlof12h，1109017pg／mlof24h。535

6、086pg／mlof48hTheresultsshowedthatESantigenisharmfultomicethymuslymphocytes，andthesensitivityincreasesalongwithtimegotng2AO／EBdoublefluorescencecolorationWaSusedtodetectapoptosisinducedbyZspiralismusclelarvaeESantigeninmi

7、cethymuslymphocytesfromthemorphologicalaspectItrevealedthatESantigeninducedlymphocytesdeathmainlybythewayofapoptosis3TheeffectofESantigenonDNAdamageinmicethymuslymphoeyteswasdetectedbythemethodofDNALadderanalysisTheresul

8、texhibitedobviousladdershapedstrapsandshowedthatESantigencausedapoptosisoflymphocytesbyDNAdamage，whichexhibitedtimedependenteffect4FlowcytometryassaywasusedtodetectcellcycleandDNAcontentsofmicethymuslymphocytes，andtheres

9、ultindicatedthatESantigeninducedapoptosisofmicethymuslymphocytesThephenomenonofZspiralismusclelarvaeESantigeninducedmicethymuslymphocytesinvitrowasdetectedinthisstudyfrommorphologyandbiochemistry，andtheresultsshowedthatE

10、Santigeninducedapoptosisofmicethymuslymphocyteswithtime—dependenteffect，therebyindicatedESantigencancauseimmunesuppressionofhostThisfindingwillplayanimportantrollinpathogenesis，preventionandtherapyofinfectionofZspiralisa