湖南岳陽柑橘黃龍病調查與PCR檢測.pdf

1、湖南農業(yè)大學碩士學位論文湖南岳陽柑橘黃龍病調查與PCR檢測姓名：王紅申請學位級別：碩士專業(yè)：果樹學指導教師：張秋明20061201InvestigatingandPCRdetectingcitrusHuanglongbinginYueyangHunanprovinceAbstractCitrusHuanglongbingc跚sedbyCandidatesLiberibacteris呲ofthemostsevcrediseasesThey

2、ieldandqualityofeffectedcjtll塔couldbede盟胄19ed∞恤thewholetreediedYueyangisonthenorthpartofHunanwhichintroducedmassivelyGuangximiyoupolemoesfromFujianProvinceonthemiddleof90’SNowthetotalplantareaofGuangximiyoupolemeesisabou

3、t200hmButinrecentyears，someGuanxiomipolemotreeswerefoundthephenomenonthatleavesbecomeyellow，fruitsizebecomesmall，evensometreeswerediedandcitrusorchardwe$destroyedThesymptomisextremelysimilarwithcitrusHuanglongbingToconfi

4、rmthereasonandruleofHuanglongbingThisresearchhascarriedonfieldinvestigationandPCRdetectingfrom2004to2006inYueyangcitrusorchardTheresultisfollows：1111esymptomoffruit。leaftrunkandbranchwhichissimilartocitrushuanglongbingwa

5、sinvestigatedandanalysisedGuanximiyoupolemocitrushavethreeleafyellowesymptomAtthefirststage，leavesononeortwoofbranchbecomeblotyelloweoryelloweThen，virusinfectedtrees’fruitbecomesmall，somefruitbecomeabnormal，andsometreesa

6、relowerthannormaltreesWeCanusethiskindsymptomtodiagnosecitrushuanglongbing2ThePCRdetectingresultwhichcomefromsweetorange，SatumaMandarin、Ponkan、navelorange、thYoupoleme、andNanfengMandarinissimilartothecontrol，fivevarieties

7、arenotinfectedbycitrusHuanglongbingvirus3TheD／印hor／a刪wasnotbefoundinYueyangcountyAccordingtoPCRdetectingandinvestigatingresult，citrusHuanglongbingCannotbedisseminatedbetweendifferentvarietiesinthesameorchard，SOthereasono

8、fHuanglongbinginGuanximiyoupomeloisgraft4AccordingtotheinvestigatingandPCRdetectingresult，thispaperproposedameasuretocontrolcitrusHuanglongspreadinginYueyangcountyKeywords：HunanlⅡovinceYueyangcoumyCi血usHuanglongbmginvesd