“清潔”載體轉(zhuǎn)化系統(tǒng)的建立與應(yīng)用.pdf

1、華中科技大學博士學位論文“清潔”載體轉(zhuǎn)化系統(tǒng)的建立與應(yīng)用姓名：姚琴申請學位級別：博士專業(yè)：生物化學與分子生物學指導(dǎo)教師：何光源20060831AbstractWheatisoneofmajwldcrops，assuchisaprimarytargetfimprovementofagronomicacteristicsbygeicengineering.Thegoalofgeicmanipulationistoachievestablet

2、ransgenicplantswithimprovementofargronomicalacteristicstominimiseitspotenticalthreattohealthanenvironment.Transfmationviaparticlebombardmenthasbeenthepredominatedmethodinwheatgeicmanipulation.Inroutinetransfmationthewhol

3、eplasistransferedtotheregenerablematerialsofwheattorecoverstabletransgenicplantsresultedintheintegrationofvectbackbonesequenceintothehostgenomealongwiththegenesofinterests.Thevectbackconesequenceisnotonlysuperfluousbutal

4、sohasthetendencytopromotearrangementbyofferingthehomogenoussequenceshotspots.Inthepresentinvestigationseveralgenecassettesweresimultaneouslytransferredintodifferentwheatvarieties，thestabletransgenicplantshavebeenobtained

5、.The“clean”fragmenttransfmationwasefficientlyestablishedoptimizedusinglineargenecassetteslackingvectbackbonesequence.Thistechniqueremovedthevectbackbonesequencesfromtransgenicplants，wasusedtoimprovetheimptantagronomicall

6、yacteristicofcrops.Firstlyweusedthescableiveremarkergenes(gusbar)todevelopoptimisethe“clean”fragementtransfmationprocedure.Stabletransgenicplantswererecoveredfromtheimmatureendospermswithtransgenesonthecircularwholeplas(