抗氧化劑在實驗性重癥急性胰腺炎對核因子-κB和環(huán)氧合酶-2的影響.pdf

1、中南大學(xué)碩士學(xué)位論文抗氧化劑在實驗性重癥急性胰腺炎對核因子κB和環(huán)氧合酶2的影響姓名：劉云申請學(xué)位級別：碩士專業(yè)：外科學(xué)指導(dǎo)教師：李宜雄20050501TheinfluenceofantioxidantsonnuclearfactorkappaBandcyclooxygenase一2inexperimentalsevereacutepancreatitisAbstractAIM：Toinvestigatetheinfluenceofan

2、tioxidantsOrlnuclearfactorkappaBandCOX一2inexperimentalsevereacutepancreatitisAndexplorethepossiblemechanismsofdecreasingseverityinSAPbyantioxidantsMETHODS：Atotalof36spraquedawley(SD)maleratswererandomlydividedintoshamope

3、rationgroups(groupA，n=12)，nonNACinterventionSAPgroups(groupB，n212)andNAC(Nacetylcysteine)interventionSAPgroups(groupC，n212)SAPwasinducedbyretrogradeinjectionof5％sodiumtraurocholate(1ml／kg)intobiliopancreaticductAndratsin

4、groupC，NACwasinjected(OOSmg／kg)1hourbeforelaparotomyintraperitoneallyGroupA，onlylaparotomywasperformedAllratsweresacrificed6hafteroperation，bloodandpancreatictissuesampleswerecollectedforassaysSerumamylase(AMS)activitywa

5、smeasuredbythebluestarchmethodandseruminterleukin1betawasdeterminedbyELISAThepathologicalchangesofpancreatictissueswereobservedandscoredundermicroscopyImmunohistochemicalSPmethodwasusedtodeterminetheexpressionofCOX一2andN

6、FKBinpancreatictissuesRESULTS：At6hourafterinductionofSAP，thesenlmconcentrationofamylaseandinterleukin1betaweremuchhigheringroupBandgroupCthaningroupA門POos)TheexpressionsofCOX一2andNF‘rd3p65proteinswerepositiveingroupBandg

7、roupC，andtheywerenegativeingroupAThelevelofCOX一2andNF—r。Bp65proteinexpressioningroupCwaslowerthaningroupB(P005)，comparedwithgroupA，thepathologicalscoresofpancreaticedema，necrosis，inflammationandhemorrhageofgroupBandgroup