腦腫瘤干細胞多藥耐藥基因表達的實驗研究.pdf

1、中南大學碩士學位論文腦腫瘤干細胞多藥耐藥基因表達的實驗研究姓名：曾飛躍申請學位級別：碩士專業(yè)：外科學（神經(jīng)外科學）指導教師：方加勝20060501碩士學位論文英文摘要Experimentalstudyoftheexpressionofmultidrugresistance(MDRl)geneinbraintumorstemcellsAbstractObjectiveThediscoveryofbraintumorstemcellshas

2、changedourviewofcarcinogenesisandchemotherapyBraintumorstemcellsmightrepresentthecelloforiginofbraintumorAlthoughchemotherapykillsmostcellsinatumoritisbelievedtoleavebraintumorstemcellsbehind，whichmightbeanimportantmecha

3、nismofresistanceTheaimofthisexperimentistoinvestigatetheexpressionofMDRlinbraintumorstemcells，andquantitatetheP—glucoseproteinwhichisdecodebyMDRlWehopetogetabetterinsightintothemechanismsofbraintumorstemcellsresistanceto

4、chemotherapy，whichmightleadtonewtherapeutictargetsandbetteranticancerstrateglesMethods(1)Tumorsampleswereobtainedfromconsentingpatients，actutelydissociated，trituratedintosinglecells，andtheninoculatedintoserum—freemediumA

5、ftertheprimarybraintumorspheresgenerated，theyweretrituratedagainandpassagedintofleshmediumFinally，theyweredigestedintosinglecellswithdispase(2)TodeterminewhetherithasMDRlexpressiononthetumorstemcellsandhowmanyithas，weper

6、formedimmunocytochemistryandimmunofluorescencetechnic(3)Atlast，weanalysedtheexpressionofpgPwhichisencodedbyMDRl，andcomparedtheresultwithdifferentiatedtumorcellsResultThebraintumorstemcellsisolatedfromneuroepithelialtumor

7、s，havetheabilitytopropagateanddifferationateinvitroThecharacterofexpressionCDl33wasprovedbyimmunocytochemistryandimmunofluorescencetechnic，thesamewiththeMDRl，andtheinfluencewhichexpressedontheBTSCwas43253％FinallythePgPwh