子癇前期患者血清對滋養(yǎng)細胞侵襲能力及平滑肌細胞凋亡的影響.pdf

1、蘇州大學碩士學位論文子癇前期患者血清對滋養(yǎng)細胞侵襲能力及平滑肌細胞凋亡的影響姓名：顏士蘭申請學位級別：碩士專業(yè)：婦產(chǎn)科學指導教師：滕銀成20100501嘲0f舯戮lar叩Sias叭啪叩叫吼r0I】hl，bl瀲捌ve出ililyand№刪lical砷ay輒刪l瞅咧!翌啦Effectofpreeclampsiaserumoncytotrophoblastinvasiveabilityandhumanumbilicalarterysmooth

2、musclecellapoptosisAbstractobjectiveToinvestigatecyto仃ophoblastinvasiveabilityandhumanumbilicalarterysmoothmusclecellapoptosisinthemodelofcyto仃ophoblastcoculture、析nlhumanumbilicalarterysmoothmusclecellinducedbypreeclamps

3、iaserumMethodschorionicvilloustissueofnormal68weekpregnancywereobtainedcytotrophoblast(CTB)andhumanumbilicalarterysmoothmusclecell(HUASMC)werecollectedbyexplantcultureconstructthemodelofCTBeoeulturewitllHUASMCandincubate

4、dwilllnormalorpreeclampsiaserunlfor24hours嬲controls，CTBorHUASMCWcIemonoculturedandtreatedidenticallytothecoculturesituationAnalysisofthecytotrophoblastinvasiveability，HUASMCactivityandapoptosis，expressionsofMMP一2，MMP一9，F(xiàn)

5、as，F(xiàn)aslmRNAandFasproteinweledetected誦也TranswellinvasionassayCCK8，flowcytometryreversetranscriptionpolymerasechainreaction(RTPCR)andWbsternblotResulUInthemodelofC113coculture謝tllHUASMC，thepreeclampsia∞r(nóng)unlcaneffectivelyde

6、creasethecytotrophoblastinvasiveabilityincreaseHUASMCactivityanddecreaseitsapoptoticrateanddecreasetheexpressionsofMMP2，MMP9，F(xiàn)as，F(xiàn)aslmRNAandFasproteinConclusionthepreeclampsiaserumaffectcytotrophoblastinvasiveabilityinam