水稻OsNAT7基因啟動(dòng)子的克隆與分析.pdf

1、首都師范大學(xué)碩士學(xué)位論文水稻OsNAT7基因啟動(dòng)子的克隆與分析姓名：汪星申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：細(xì)胞生物學(xué)指導(dǎo)教師：黃勤妮20080405首都師范大學(xué)碩士學(xué)位論文AbstractInpreviousresearch，ourlabacquiredagene(OsNAT7)whichhasthehighestratioof適elFeinricerootsbasedonthemicroarrayanalysisToelucidateitsre

2、gulatorymechanismatthetranscriptionallevel，OsNAT7promoterhasbeenclonedandthesequencehasbeenanalysisedbybioinformatictoolsThenthepromoteractivityintransgenictobaccoBY2cellsuspensionhasbeencharactefizatizedinordertoprovide

3、ainsightintotheroleofOsNAT7geneTwoputativecisactingelements：IDEllikeandIDE2likehasbeenfoundinOsNAT7promoterToidentifytheirfunction，15kbfragmentofOsNAT7promoterhasbeenamplifiedbynestPCRmethodOsNAT7p：：GUShasbeenconstructed

4、respectivelyandtransferredinticecallusesbyparticlebombardment，successfulexpressionofGUSgenewasobtainedToidentifytheputativeirondeficiencyresponsiveregion，deletionsfromthe5endhasbeentransformedintotobaccoBY2cellsuspension

5、protoplastbyPEG—mediatedmethodTransientexpressionrevealedthatOsNA17promoterdiden’trespondtoirondeficiencyintobaccoBY2cellToelucidatetheexpressionpatternofOsNA17intissue，plantexpressionvectorpCamOsNAT7phasbeentransformedi

6、ntotobacoo，riceandtobaccoBY2cellsuspensionbyagrobacterium—mediatedmethodToseedlingsoftransgenictobaccos，transgenicricecallusesandtransgenicBY2tobaccocellsuspensionwasobtainedInducibleexpressionpatternofOsNAT7p：：GUSintran

7、sgenictobaccoBY2cellsuspensionwasanalyzedTheresultsindicatedthatthepromoteractivityofOsNAl7wasnotrelativetoiron、】rnanganese＼copperkzinc，butcanbeenhancedbycitricacidCitricacidCanfacilitatetheuptakeandtransportofiron，littl

8、ehasknownaboutthetranscriptionalregulationinresponsetocitricacidFurtherbioinformaticanalysiscombinedwithmicroarrayresultsindicatedthattheexpressionofOsNAT7genewaspossiblelyregulatedbyothergenewhichcanrespondtoirondeficie