谷胱甘肽-S-轉移酶P1基因多態(tài)性及其與奶牛熱應激關聯(lián)性研究.pdf

1、四川農(nóng)業(yè)大學碩士學位論文谷胱甘肽S轉移酶P1基因多態(tài)性及其與奶牛熱應激關聯(lián)性研究姓名：廖坤申請學位級別：碩士專業(yè)：動物遺傳育種與繁殖指導教師：賴松家仲躋峰20080601AbstractGlutanlioDeStransfemsePi(GSTPDisallinteriorlyimportantdetoxicatedenzymeofmammals，involvinginthemaintainingbalanceofredoxstateRe

2、searchshowedthatglutathione。Strans融稻eincellsWaSinvolvedinoxidativestress蝎uryintheprocesswhencellswerestimulatedbytheheat，removingtheheatstressfreeradicalsWechooseGSTPIgoneaScandidategonewhichaffectOrcontralheatstressofdr

3、aiyCOWTheaSsociationbetweenbovineheats仃essinjuryandSNPswhichusedsequencingofGSTPlgonewaSstudiedTheresultswere觴foUows：ThecompletesequenceofGSTPlgonewassequgnced，is2869bp35SNPsofGSTP1gonew笛dotectedin2cattlebreedsincluing30

4、HosteindraiyCOWand15LuXiyellowcattle19of35SNPswerereportedbyUSpartoftheSNPslocatedinintron，SNPslocatedinCDSbelongtosilentmutationexceptforA2696Gresultinginchangesofaminoacid(V181M)inLuXiyellowcattle3SNPsincludingG17C，T32

5、6CandA2696GWaSstudiedbyPCRRFLPin120hosteindraiycowItisshowedthat2allele(denotedasGandC)and3genotype(denoted酶GG，GCandCC、w弱dotectedin017Cloci，the丘equencyof2alleleand3genotypeWaS04791，O5209，02583，04417，03000respectively2all

6、ele(denoted∞MandN)and3genotype(denoted笛MM，ⅢandmwasdetectedinT326Clocithefrequencyof2alleleand3genotypeWas08917，01083，08167，O150000333，respectivelyThereisnotdigestionpolymorphisminA2696Gloci，but2allelewerefoundinLuXiyello

7、wcattlepopulation，andthefrequencyof2allelewas01286and08714GLManalysisfortheeffectofgenotypesonindexofheattorlerance(aIT)demonslratedthatdifferectgenotypesWaSnotsignificantlyaSsociatedwithrectaltemperatureandpotassil21／1c

8、oncentrationofredbloodcells(PO05)TissueexpressiondifferenceofGSTP1goneundernormalandseriourheatstressconditionWasdetectedbyRTPCRAnalysisshowedthatGSTPIgoneWaSexpressedinheart，lung，kidneyandspleenundernormalandheatstressc

9、ondition，andthereWaSnotsignificantdifferenceindifferenttissue(P005)However，thereWassignificantdifferenceinliverundernormalandseriourheatstresscondition(Po05)KEYWORDS：HolsteinCOWS；GSTPlHeatstress；SequenceAnalysis；GoneExpr