攜帶Il-24基因的條件增殖型腺病毒聯(lián)合紫杉醇誘導(dǎo)乳腺癌細(xì)胞凋亡的研究.pdf

1、徐州醫(yī)學(xué)院碩士學(xué)位論文攜帶IL24基因的條件增殖型腺病毒聯(lián)合紫杉醇誘導(dǎo)乳腺癌細(xì)胞凋亡的研究姓名：戚迎冬申請學(xué)位級別：碩士專業(yè)：腫瘤學(xué)指導(dǎo)教師：鄭駿年201203徐州醫(yī)學(xué)院碩士學(xué)位論文EnhancedantitumoractiVitybyaconditionallyreplicatingadenoviruscarryin2Interleukin24combinin2withaden0VlrUSCarrVln2Interleukin—Z4C

2、0mbiningwithpaclitaxelfbrbreasttumorcelllinesviainductionofapoptosisAbstractobjectiVeToinVestigatethetefIfectsofthecombineduseofconditionallyr印licatingadenoViruscarr如ngIL一24(ZD55一IL一24)incombinationwithPTXonthe黟。叭ha11dap

3、optosisofbreastcancercelllinesMDA—MB231andBcap37Methods‘TlleVimswerepropagatedin10wpassageHEK293cellsTllemnctionalPFUtitersweredetemlinedbyplaqueassayonlowpassageHEK293cellsMDA—MB一231andBcap37cellsweretreatedwithPBS，ZD55

4、一EGFP，PTX，ZD55一IL24，ZD55EGFPplusPTXandZD55IL24plusPTXrespectivelyTheihhibitionofMDAMB231andBcap一37cellpr01iferationwasdetenninedbyCCK8assayThec”otoxiceffectsweredetectedbyHoechst33258andAnnexinVFITC／PIFurthenllore，IL一24，

5、Bax，Bcl一XL，PARP’Mcl一1，Caspase一3，Caspase一7，Caspase9proteinsweredeteminedbyWestemB10tResultsThe如nctionalPFUtitersofZD55一IL一24ZD55一EGFPwere65108p如／m1，82106pn_l／m1CCK8assayshowedZD55一IL24plusPTXcouldinduceobViouslygrowthinhi

6、bitionofMDA—MB一231andBcap37cellsHochest33258andAnnexinVFITC／PIstainingindicatedobviousapoptosisoftheMDAMB231andBcap一37cellsWestemBlotanalysisconfinnedthatZD55一IL一24canexpressIL一24inMDAMB一231andBc印37cellslinewithhighe瓶cie

7、ncyconcomitantwithincreasingproapoptoticproteinsBax，down—regulatingtheantiapoptoticproteinsPARP，Mcl1andBcl—XL，aswellas，actiVationofCaspase一3，Caspase7，Caspase一9ConclusionThisstudyshowedthatzD55IL一24conjugatedwithPTxexhibi

8、tedaremark￡lblyincreasedc舛otoxicandapoptosis—inducingeffecIinbreastcancercellsThischemo—geneVirotllerapeuticstrategyissuperiortotheconventionalchemotherapyorgene—virotherapyaloneKeywordsBl’eastcancel；IL一24；Conditionallyr