雪旺細(xì)胞對大鼠視神經(jīng)保護(hù)機(jī)制的實驗研究.pdf

1、福建醫(yī)科大學(xué)碩士學(xué)位論文雪旺細(xì)胞對大鼠視神經(jīng)保護(hù)機(jī)制的實驗研究姓名：湯疇旺申請學(xué)位級別：碩士專業(yè)：眼科學(xué)指導(dǎo)教師：朱益華20090401ExperimentalstudyabouttheprotectionmechanismofschwanncellsfortheratopticnerveAbstractObjective：ToobservetheeffectofSchwanncellsONtheexpressionofciliaryn

2、eurotrophicfactor，andexploretheprotectionofSchwanncellsontheretinalganglioncellsMethods：ByDual—enzymedigestionmethodisolatedandcultured，purifiedratSchwanncells；theintravitreouswasinjectedwithSchwanneel1s；threedayslaterto

3、establishtheacutehighintraocularpressuremodelbytheanterioreharaberperfusion；Aflersuccessfullyestablished，usingRTPCRtodetemtheexpressionsofciliaryneurotrophicfactormRNAintheratretinaatdifferentstagesofthe1stday3rdday7thda

4、y14thdayand21thda)，；bytheTwostepimmunohistochemicalmetheodtodetectthedifferentexpressionlevelofproteinResuRs：SuccessfullyisolatedandculturedratSchwannce11s；establishedallacutehighintraocularpressuremodelRTPCRresultsshowe

5、dthattheciliaryneurotrophicfactormRNAexpressionwasgratuallyenhancedafteracutehighintraocularpressureinjurywhileweaklyinnormalratretinatissues，andreachedthepeakexpressionlevelinthe37thdaysoftheinjurythenbegantodecrease，ci

6、liaryneurotrophicfactormRNAexpressionchangesinDMEMinterventiongroupweresimilartotheacutehypertensiongoup，intheSchwanncellsinterventiongrouptheciliaryneurotrophicfactormRNAexpressionWaShigherthanbothintheacutehypertension

7、goupandtheDMEMinterventiongroupateachstage，thereweresignificantdifferences(pO05)TheImmunohistochemicalstainingmatchedwithPT—PCRexpressioninretinaslices，Positivestainingproductpositioningshowed，ganglecelllayerhadlittlecil

8、iaryneurotrophicfactorpositiveproductexpressioninnormalratretinaandinceasedafteracuteocularhypertensioninjuryCi1iaryneurotrophicfactorpositiveproductscouldbefoundinganglecelllayernervefiberlayerandinnernuclearlayerCi1iar