用于快速檢測二噁英類化合物的QCM生物傳感器的構建及應用.pdf

1、天津大學碩士學位論文用于快速檢測二噁英類化合物的QCM生物傳感器的構建及應用姓名：趙勇申請學位級別：碩士專業(yè)：環(huán)境工程指導教師：劉憲華201112ABSTRACTQuartzcrysta4mierobalance(QCM，QuartzCrystalMicrobalance)sensorhasthecharacteristicsofIesponsetoabroadspectrum，highsensitivity，andsimplestru

2、cture，lowcostdetection)devices，especiallyasaquality—basedsensorsarewidelyusedinanalyticalchemisl時surfacechemistrybiochemistryandenvironmentalmonitoringandmanyotherfi61dsSurfacesensitivefilmisusedassensitivecomponentsinQC

3、MsensorsandtheA％cutquartzcrystalastransducerelementsWeusetherelationshipofquartzcrystalqlualitychangefrequency；transferthequality，concentrationsignalintofrequencyTherebyachievethepurposesofdetectingthequalityandconcentra

4、tionThepurposeofthispaperistodevelopsensorsfordetectingtraceconcentrationsAn8MHzATcutquartzcrystalresonatorwasmodifedwithreceptorandantibodyWehaveusedthattensortodetecthumanserumalbumin(HSA)and2，3，7，8TCDDintealtimeMainco

5、ntentsfindresultswereasfollows：Firstlytheprinciple，classificationandapplicationofsensorswereintroducedTheprincipleofpi—ezoelectricquartzcrystalanddetectionmethodsofdioxinwereconsideredWeintroduceanewtechnologytodetectdio

6、xinbyusingQCMSecondly，weoltainedtheexperimentalconditionstoensurethestabilityofthesystemthroughmalnyexperimentsonthetemperatureandflowrate，thoseareconstanttemperatureandthemaximumflowrateof70Itl／minFinallywetestedthesyst

7、emperformancethroughglycerinsolutionofdifferentconcentrationsthatwere5％、10％、20％、30％、40％ThirdlyselfasLsembledmonolayerwasdiscoursedTheselfassembledmonolayertechnolog【yandcharacterizationmethodsweredescribedLipoicacidwasch

8、osetoformselfa；semblymonolayerandhighinsulatingpropertiesoftheacidicthiolmonolayerhabeencharacterizedwithcyclicvoltammetryandscanningelectronicmicroscop)tFourth，abiosensorbasedonaquartzcrystalmicrobalance(QCM)wasdevelope

9、dfordynamicandrealltimedetectionofHSAThefrequencyresponsesduetotheadsorptionofthestahdardsamplesonthebiosensorsurfaceweremeasuredTheresultsshowthatHSAcanbequantitativelydetectedwiththedevelopedimmunosensorinthedoncentrat