鹽環(huán)境細(xì)菌群落結(jié)構(gòu)分析和多相分類學(xué)研究.pdf

1、浙江大學(xué)生命科學(xué)學(xué)院博士學(xué)位論文鹽環(huán)境細(xì)菌群落結(jié)構(gòu)分析和多相分類學(xué)研究姓名：吳月紅申請(qǐng)學(xué)位級(jí)別：博士專業(yè)：微生物學(xué)指導(dǎo)教師：吳敏20090501浙江大學(xué)博士學(xué)位論文鹽環(huán)境細(xì)菌群落結(jié)構(gòu)分析和多相分類學(xué)研究AbstractHalophilicmicroorganismsaremicrobesinhabitedindiversesalineenvironmentsAccordingtotheiradaptationtosaltsuppleme

2、ntedinmedia，thesemicrobesaredividedintohalophilicandhalotolerantgroupsThehalophiliemicroorganismscangrowuptothehighsaltconcentrations，suchassaltlakes，sodalakes，solarsaltems，hypersalinesoils，marineenvironments，etc，andthey

3、oftenhavestrongimpactontheecosystemsinwhichthethriveTl艙propertyofmicrobialadaptationtohighsaltenvironmentsismainlyrelationwithenzymesandotherbio-activesubstanceMuchinterestinhalophilicadaptationhasbeensupportedbythesearc

4、hforlifeontheplanetsandtheapplicationsinbiotcchnologySoitisverysignificantnotonlyintheorybutalsoinpracticetostudyhalophilesMyresearchisbasedonourlab’Sworksuchassamplescollectionfromhi【曲saline，microorganismscultivationand

5、relatedmoleculartechnologyAyakekumuLakelocatedinXinjiangregion，theestuaryoftheYangtzeRiveroftheEastChinaseaandtheChinanoduleprovinceinEastPacificoceanwhichwerecollectedfromthreeenvironmentecosystemswereinvestigatedCultiv

6、ation-independentapproachesbasedonrRNAcloningandsequencingwereappliedtosurveymicrobialdiversityinnaturalecosystemsTheidentificationandbiodiversityofculturablemicroorganismswereperformedbyusingthemoleculartechniquesubsequ

7、entlyBasedontheanalysisof16SrDNAgenesequenceanalysisandphenotypecharacteristics，thedatabaseofhalophileswasestablishedandenrichedInthisstudyusingmolecularbiologymethods，construct16SrDNA，napA，n／rSbiomarkergenesclonelibrari

8、esattheestuaryoftheYangtzeRiveroftheEastChinaseaTotal366cloneswereanalysisand256clonesweresequencedandphylogeneticanalysedBacteriabiodiversitywasveryrich，mainlycomposedby7-Proteobacteria，a-Proteobacteria，Acidobacteria，6一

9、ProteobacteriaandBacteroidetesItindicatemicrobialcommunitystructureandtheirpossiblegeochemicalprocessInaccordancewiththeprincipleofpolyphasictaxonomy,asystematicstudyhadbeenconductedonthetaxonomyofeightisolatesSixnovelmo

10、deratehalophilicbacteriaspecies(Halomonascaseinilytica,Halomonassalifodinae,MarinobacterPelagius,Pseudidiomarinamaritima，Pseudidioraarinadon#aiensis,Hbriohangzhouensis)aswellasonehalotorleranthalophilicbacteriaspecies(Mi