Nd3+和Sm3+對(duì)原代培養(yǎng)的成骨細(xì)胞增殖、分化和礦化功能的影響.pdf

1、河北大學(xué)碩士學(xué)位論文Nd3和Sm3對(duì)原代培養(yǎng)的成骨細(xì)胞增殖、分化和礦化功能的影響姓名：尚密芹申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：應(yīng)用化學(xué)指導(dǎo)教師：張金超201105AbstractⅡAbstractAseriesofmethodsincluding3(45dimethylthiazol2yl)25diphenyltetrazoliumbroe(MTT)methodalkalinephosphatase(ALP)activitymeasurement

2、OilRedOstainmeasurementmineralizedfunctionquantitativerealtimePCR(qPCR)wereemployedtoassesstheeffectofNd3Sm3ontheproliferationdifferentiationmineralizedfunctionofprimaryosteoblasts(OBs)invitroatcellmolecularlevels.Theres

3、ultsshowedthat:Nd3Sm3suppressedtheproliferationofOBsattestedconcentrationsmeovertheinhibityeffectofSm3wasstrongerthanthatofNd3.AstotheALPactivityNd3Sm3showedsimilareffectstheyincreasedtheALPactivityatahigherconcentration

4、of1105molL.HowevertheALPactivitywassharplydecreasedataconcentrationof1106molLgraduallyincreasedasconcentrationdecreasingtheyturnedtoincreasetheALPactivityataconcentrationof1108molL.Nd3promotedthefmationofmineralizedmatri

5、xnodulesatahigherconcentrationof1105molLinhibitedthefmationofmineralizedmatrixnodulesatconcentrationsof11071106molLbuthadnoeffectataconcentrationof1108molL.Sm3inhibitedthefmationofmineralizedmatrixnodulesofOBsattestedcon

6、centrations.Nd3Sm3exhibiteddistinctiveeffectsontheadipogenictransdifferentiationofOBs.Sm3(110711061105molL)promotedtheadipogenictransdifferentiation.Nd3suppressedtheadipogenictransdifferentiationattestedconcentrations.Me

7、overweobservedthegeneexpressionofruntrelatedtranionfact2(Runx2)peroxisomeproliferatsactivatedreceptγ(PPARγ)duringdifferentiationofOBs.TheexpressionofthemRNAfRunx2wassignificantlydownregulatedinthepresenceof1106molLSm3Nd3

8、.TheexpressionofthemRNAfPPARγwasslightlydownregulatedby1106molLNd3butsignificantlyupregulatedby1106molLSm3.OurfindingssuggestedthattheeffectsofNd3Sm3ontheproliferationdifferentiationmineralizationfunctionofprimaryOBsinvi