ERF蛋白TERF1調(diào)控?zé)煵菀蚁┥锖铣刹⑻岣邼B透脅迫耐性的分子機理.pdf

1、中國農(nóng)業(yè)大學(xué)博士學(xué)位論文ERF蛋白TERF1調(diào)控?zé)煵菀蚁┥锖铣刹⑻岣邼B透脅迫耐性的分子機理姓名：張執(zhí)金申請學(xué)位級別：博士專業(yè)：細(xì)胞生物學(xué)指導(dǎo)教師：王學(xué)臣黃榮峰20070501AbstractERFs(_EthyleneResponsiveF_actors)arespecificfamilyoftranscriptionfactorsinplantsERFsact∞thedownstreamcomponentofethylenesign

2、alingpathwayand端oulatetheexpressionofPR(Pathogenesis_Related)g∞％throughbindingtoCCCboxandincreasetheresistanceofplanttopathogenRecently80mestudiesdemonstratethatERFs∞alsoinvolveinotherstressesR8lmnsepathwaysincludinglowt

3、emperaturedroughtandhighsaltaadABAMeJASATheseresultsindicatethatERFsmayphykeyrolesinmcdiatingtheinteractionofdifferentplantsignalingpathwaysHoweverthemechanismofERFsinregulatingtheinteractionbefwealdifferentplantstre％res

4、ponsesignalingpathwaysisnotwellunderstoodThegeuecodingERFproteinTERFl(!omatoE_thyleneResponsiveEactorDwasisolatedfromtomatoeDNAlibrarybyyeastonehybridsystemourpⅢiousexperimentsshowedthatTERFIcmactasatranscriptionalactiva

5、torandactivatetheexpressionofPRgenesintransgenictobaccoHerewefllrthefanalyzedthemechanismofTERFIeahancingtheosmoticstresstoleranceOre“resultsdemonstratedthatTERFIminteractivithbothGOCboxaadtheDREelememtoactivatetheexpres

6、sionofstRssrdatedgenesregulatedbyGCCboxOfDREelementandcnh∞∞thetoleranceofs打essbyimprovingtheplants燈e齬esrespame啪latedmetaboficactionTheoverexpvessionTERFItobaccodisplaysthephenotypeofethyleneo、，a[prodI|ctiomTheWansgenicto

7、baccoetiolatedseedlingshowesthetypicpheaotypeof“uipleresponse“FurtherstudiesillustratedthatTERFImayactivatetheexpressionofethylenebiosynthesisrelatedgenes，includingACS3，ACOISubsequentExperimentshowedthatTERF!∞ndirectlyre

8、gulatetheexpressionofACS3throuOlbindingtoGCCboxinthepromoterTheseresultsindicatedthathighexpressivelevelofethylenesynthesisrelatedgenesmayresultintheoverproductionofethyleneinUansgenictobaccohadditionWOanalyzedtheinterac

9、tionofABAandethylenesignalingpathwayinTERFlmediatedNaCIstressm印∞％OurstudiesillustratedthatbothABAandethylenesignaIpathwaysaffectTERFItoregulartheresponseofosmodc6tressTheinhibitorofethyleneandABAsynthesisoractivationmaff

10、ecttheexpremooofgenesregulatedbyTERFICombiningourpⅪio=studiesthatthea【plⅨs湎ofTERFIisinducedbyethylerieABANaCIcoldOrtroughtreatmems，OUt“resultsindicatedthatTERFImayregulatetheexpressionofosmoticstressrelatedgC／1CSandenhac

11、esthetoleranccofosmotics嘧byABAandeihylenesignalpathwayThereiscomplexrelationofABAandethylenesignalingpathwayduringsn％sRspalse，whiIeTERFIplayanimportantflakingroleduringtheresponsecourseOurstudieswillhelpunderstandthefunc