杠柳根皮化學成分及生物活性的研究.pdf

1、Y833856分類號：Q94683UDc：81：塑密級：公開級學校代碼110712研究生學號：TG02178西弛農林科教大學、。2005屆在職攻讀碩士學位研究生學位論文杠柳根皮化學成分及生物活性的研究類型：農業(yè)推廣領域、專業(yè)、方向：種植研究生：史清華指導完成教師：時間：馬養(yǎng)民副教授二00五年十月中國陜西楊凌ABSTRACTASTUDYONCHEMICALCOMPOSITIONANDBIOACTIVITYOFRoOTBARKFROM只E&

2、滬己D(■S!E阿(刀MABSTRACTPeriplocasepeiumBgebelongstoAsclepiadaceaefamilyanddistributeswidelyinChiIlaInshaanxi’province，theplantchieflygrowthsinQin—Bamountain，Guanshanmountain，HuanglongmountainandQiaoshanmountainandhasplentyo

3、fresourceInorder，toexploitandusetheplantresource，westudiedthechemicalcompositionandfarmfunction●ontherootbarkofPeriplocasepiumApreliminarystudyonchemicalcompositionofrootbarkfromPeriplocasepiumWasaccomplished。ItiSindicat

4、edthattherootbarkcontainsaminoacidscarbohydrateanditsglycosides，organicacids，saponins，alkaloid，phenols，cardiacglycosides，lactones，coumarinsanditsglycosides，sterols，triterpenes，volatileoil，fatsAlkaloidofthemiSfirstdetermi

5、ned。intheplantMeanwhile，Extractsofpetroleum，ethylacetate，n—butylalcoholandwaterfrommethanolcrudeextractsoftherootbarkwereusedtotesttheinsecticidalactivityagainstSchizaphisgraminum(Rond)andMythimnaseparata(Walker)Theresul

6、tsshowedthatnbutylalcoh01extractspossessobviouscontactpoisonagainstSchizaphisgraminum(Rond)andcertainstomachpoisonagainstMythimnaseparata(Walker)，ThechemicalcompositionofthevolatileoilfromPeriplocasepiumrootbarkwasanalyz

7、edbymeansofgaschromatography—massspectrometer(GC／MS)technique10compoundsof15componentsseparatedfromthevolatileoilwhichconstitute9491％thetotaloil，wereidentifiedtheyare4methoxysalicytaldehyde，butylacetate，2methyl—l，3一dioxy

8、—cyclopentyl—ethylacetate，S[8一(diethylphosphono)octyl】ethanethioate，1，1，3，3，一tetrabutoxy一2一propanone，4methyl一2pentanone，butylfonrtate，1一(1ethoxyethoxy)一butane，2butoxytetrahydropyran，butyletherMeanwhile，theresultoftestoni

9、nsecticidalactivityofthevolatileoilaccomplishedshowedthattheoilpossessesobviouscontactpoisonagainstSchizaphisgraminumFourextractsofpetroleumether，ethylacetaten—butan01andwaterwereobtainedfromthemethanolextractoftherootba

10、rkinPeriploeasepeiumCompoundIandcompoundsII，IIIIVwereisolatedrespectivelyfrometheracetateextractandn—butanolextractbycolumnchromatographyofsilicagel，C18andSephadex—LH一20ThefourcompoundswereidentifiedasB—amyrinacetate，4me

11、thoxysalicylaldehydelactoside，periplocosideEandacardiacglycosidewithchemicalmethodandspectralanalysis(IR，。H剞MR，”CNMR，El—MS)Meanwhile，compoundsII，Ili，IVwereusedtotestOUbioactivityTheresultsshowedthatIIrestrainwheatandrape